PMID: 9163900May 1, 1997Paper

Rapid distinction between Leptospira interrogans and Leptospira biflexa by PCR amplification of 23S ribosomal DNA

FEMS Microbiology Letters
T H WooB K Patel

Abstract

Bacterial specific primers were used to amplify 23S rRNA genes from a representative strain from each of the 23 serogroups of the pathogenic Leptospira interrogans and 8 strains from 6 serogroups of the non-pathogenic Leptospira biflexa. Only regions of extreme variability, which had been identified on the basis of homology-based search of all the 23S rRNA sequences available in GenBank database, were sequenced from the amplified products. PCR primers that had the potential to distinguish L. interrogans from L. biflexa species were designed from the derived sequences and a sensitive PCR protocol developed. The PCR method enabled the differentiation of the 59 strains of the 23 serogroups of L. interrogans from the 8 strains of 6 serogroups of L. biflexa. Further investigation by 16S rDNA sequencing of two strains of L. interrogans, which gave unexpected PCR results, provided evidence that they had been misclassified and hence we propose to reassign them to L. biflexa.

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Citations

Apr 9, 2001·Clinical Microbiology Reviews·P N Levett
Dec 14, 2011·Journal of Clinical Microbiology·Nicola DecaroCanio Buonavoglia
Apr 5, 2011·The American Journal of Tropical Medicine and Hygiene·Patrick HochedezAndré Cabié
Nov 23, 2006·Environmental Geochemistry and Health·Joseph E BunnellWilliam H Orem
Sep 18, 2014·Revista Do Instituto De Medicina Tropical De São Paulo·Angel Alberto NodaAna Margarita Obregón
Oct 6, 2006·Diagnostic Microbiology and Infectious Disease·Uraiwan KositanontPimjai Naigowit
Apr 19, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Albertine LéonRoland Leclercq

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