PMID: 8586123Oct 1, 1995Paper

Rapid drug susceptibility testing of Mycobacterium tuberculosis using conventional solid media

The European Respiratory Journal
T SchabergH Mauch

Abstract

Radiometric methods for M. tuberculosis drug susceptibility testing yield much faster results than standard techniques; however, these methods require sophisticated equipment and are expensive. We investigated a rapid drug susceptibility testing method for isoniazid, rifampin, ethambutol, streptomycin and pyrazinamide in specimens from 197 patients with pulmonary tuberculosis using a simplified agar-dilution method. Middlebrook 7H11 agar solid medium and microcolony detection were used to test sputum from 64 smear-positive, and from 70 culture-positive but smear-negative patients. Culture-positive material from bronchoscopy, surgical biopsy, pleural fluid or gastric fluid of 63 patients was tested. In 64 smear-positive patients, the median time for final susceptibility results was 11 days (95% confidence interval (95% CI) 10-12 days) compared to 62 days (95% CI 56-66 days) with the standard method. In 133 smear-negative patients, results were available after a median of 35 days (95% CI 32-40 days) in contrast to 72 days (95% CI 62-83 days) with the standard method, regardless of whether or not sputum or other materials were used for primary culture. The rapid method detected all cases of single-drug resistance (n = 20) and mult...Continue Reading

Citations

May 21, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Michael L Wilson
Dec 18, 2012·Lung India : Official Organ of Indian Chest Society·Nicolas SchönfeldHolger Rüssmann
Jun 1, 2013·Archives of Pathology & Laboratory Medicine·Michael L Wilson
Jan 20, 2004·Tropical Medicine & International Health : TM & IH·Richard J Coker
Jan 7, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Md A GaziZ Mahmud
Jun 27, 2018·Antimicrobial Agents and Chemotherapy·Paola M V RancoitaUNKNOWN CRyPTIC Consortiumfor the CRyPTIC Consortiumfor the CRyPTIC Consortium
Jul 11, 2012·Annals of Laboratory Medicine·Won-Jung KohNam Yong Lee
Jan 20, 2004·Journal of Medical Microbiology·Srinivas V RamaswamyEdward A Graviss
Jun 24, 1999·Acta Microbiologica Et Immunologica Hungarica·L Manczinger
Oct 5, 2007·Expert Review of Anti-infective Therapy·Alberto MatteelliMario Raviglion
Jul 7, 2018·African Journal of Laboratory Medicine·Annie ZhangJordi B Torrelles
Sep 5, 2020·Yeungnam University Journal of Medicine·Jong Geol Jang, Jin Hong Chung

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