Rapid Dynamics of Signal-Dependent Transcriptional Repression by Capicua.

Developmental Cell
Shannon E KeenanStanislav Y Shvartsman

Abstract

Optogenetic perturbations, live imaging, and time-resolved ChIP-seq assays in Drosophila embryos were used to dissect the ERK-dependent control of the HMG-box repressor Capicua (Cic), which plays critical roles in development and is deregulated in human spinocerebellar ataxia and cancers. We established that Cic target genes are activated before significant downregulation of nuclear localization of Cic and demonstrated that their activation is preceded by fast dissociation of Cic from the regulatory DNA. We discovered that both Cic-DNA binding and repression are rapidly reinstated in the absence of ERK activation, revealing that inductive signaling must be sufficiently sustained to ensure robust transcriptional response. Our work provides a quantitative framework for the mechanistic analysis of dynamics and control of transcriptional repression in development.

References

Jan 15, 2000·Insect Molecular Biology·A M Handler, R A Harrell
Dec 2, 2004·Molecular and Cellular Biology·Charles E FouldsBarbara J Graves
Dec 22, 2005·Nature Biotechnology·Jean-Denis PédelacqGeoffrey S Waldo
May 23, 2006·Current Biology : CB·Jonathan R ChubbRobert H Singer
Jan 27, 2007·The EMBO Journal·Sergio AstigarragaGerardo Jiménez
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Johannes BischofKonrad Basler
Jun 24, 2008·Current Biology : CB·Mathieu CoppeyStanislav Y Shvartsman
Sep 19, 2008·Genome Biology·Yong ZhangX Shirley Liu
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Dec 5, 2009·Proceedings of the National Academy of Sciences of the United States of America·Ulrike LöhrHerbert Jäckle
Apr 25, 2012·Journal of Cell Science·Gerardo JiménezZe'ev Paroush
Jun 30, 2012·Nature Methods·Johannes SchindelinAlbert Cardona
Nov 6, 2012·Gene·Shen-Hsi YangAlan J Whitmarsh
May 28, 2013·Genetics·Scott J GratzKate M O'Connor-Giles
Jun 5, 2013·Proceedings of the National Academy of Sciences of the United States of America·Bomyi LimStanislav Y Shvartsman
Aug 21, 2013·PLoS Computational Biology·Michael LawrenceVincent J Carey
Jul 6, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jacques P BothmaMichael Levine
Jul 9, 2014·Proceedings of the National Academy of Sciences of the United States of America·Fillip PortSimon L Bullock
Jan 9, 2015·PLoS Genetics·Marta ForésGerardo Jiménez
Jun 23, 2015·Current Biology : CB·Bomyi LimStanislav Y Shvartsman
Jul 1, 2015·Proceedings of the National Academy of Sciences of the United States of America·Alan S FutranA James Link
Nov 22, 2016·Nature Genetics·Ross A OkimotoTrever G Bivona
Jan 25, 2017·Developmental Cell·Heath E JohnsonJared E Toettcher
Feb 7, 2017·Nature Genetics·Yogesh GoyalStanislav Y Shvartsman
Jul 1, 1986·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Trudi Schüpbach, Eric Wieschaus
Jan 6, 2018·Genetics·Ethan BierJill Wildonger
Feb 13, 2018·Proceedings of the National Academy of Sciences of the United States of America·Aikaterini PapagianniGerardo Jiménez
Jul 22, 2018·Developmental Biology·Yogesh GoyalStanislav Y Shvartsman
Jul 28, 2018·Development·Aleena L Patel, Stanislav Y Shvartsman
Feb 10, 2019·Nature Communications·Severa BundaKenneth Aldape
Feb 13, 2019·Developmental Cell·Heath E Johnson, Jared E Toettcher

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Citations

Mar 11, 2021·Developmental Cell·Caroline A DohertyStanislav Y Shvartsman
Jan 22, 2021·Molecular Biology of the Cell·Robert A MarmionStanislav Y Shvartsman
Jun 25, 2021·Current Biology : CB·Aleena L PatelStanislav Y Shvartsman
Mar 17, 2021·Annual Review of Biomedical Engineering·Payam E FarahaniJared E Toettcher
Aug 17, 2021·The EMBO Journal·Ranjith ViswanathanStefano De Renzis
Jul 25, 2020·Current Biology : CB·Heath E JohnsonJared E Toettcher
Nov 14, 2021·Proceedings of the National Academy of Sciences of the United States of America·Marianne BauerWilliam Bialek

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