Rapid effects of phorbol ester on platelet shape change, cytoskeleton and calcium transient

FEBS Letters
K YoshidaV T Nachmias

Abstract

Platelets are rapidly activated by several agonists. When phorbol 12-myristate 13-acetate (PMA) was added to washed platelet suspensions 10 s prior to either thrombin or ADP, it caused a dose-dependent inhibition of shape change correlated with decreased myosin association with the cytoskeleton and with inhibition of the calcium transient measured in fura-2-loaded platelets. PMA added 5-10 s after agonists did not reverse shape change or the association of myosin with the cytoskeleton, but markedly increased the rate at which the calcium signal returned to the baseline. The analogue, 4 alpha-phorbol didecanoate did not cause these effects. Our results suggest that one effect of C-kinase activation is to provide negative feedback in sequential responses.

References

Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·G B ZavoicoM B Feinstein
Jan 3, 1985·Nature·V T NachmiasS Jacubowitz

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Citations

Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·J T BonnerK Y Gregg
Dec 14, 1999·The Journal of Biological Chemistry·K CroceD A Potter
Mar 1, 1991·The Journal of Cell Biology·F StarkV T Nachmias
Jan 1, 1989·The American Journal of Physiology·J A WareE W Salzman

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