PMID: 7537324Jan 1, 1995Paper

Rapid electrogenic sulfate-chloride exchange mediated by chemically modified band 3 in human erythrocytes

The Journal of General Physiology
M L Jennings

Abstract

One of the modes of action of the red blood cell anion transport protein is the electrically silent net exchange of 1 Cl- for 1 SO4= and 1 H+. Net SO4(=)-Cl- exchange is accelerated by low pH or by conversion of the side chain of glutamate 681 into an alcohol by treatment of intact cells with Woodward's reagent K (WRK) and BH4-. The studies described here were performed to characterize the electrical properties of net SO4(=)-Cl- exchange in cells modified with WRK/BH4-. The SO4= conductance measured in 100 mM SO4= medium is smaller in modified cells than in control cells. However, the efflux of [35S] SO4= into a 150-mM KCl medium is 80-fold larger in modified cells than in control cells and is inhibited 99% by 10 microM H2DIDS. No detectable H+ flux is associated with SO4(=)-Cl- exchange in modified cells. In the presence of gramicidin to increase the cation permeability, the stoichiometry of SO4(=)-Cl- exchange is not distinguishable from 1:1. In modified cells loaded with SO4=, the valinomycin-mediated efflux of 86Rb+ into an Na-gluconate medium is immediately stimulated by the addition of 5 mM extracellular Cl-. Therefore, SO4(=)-Cl- exchange in modified cells causes an outward movement of negative charge, as expected for an...Continue Reading

References

Jun 1, 1975·The Journal of General Physiology·R B GunnD C Tosteson
Aug 26, 1976·The Journal of Membrane Biology·M L Jennings
Mar 1, 1977·The Journal of General Physiology·P A KnaufA Rothstein
Sep 1, 1977·The Journal of General Physiology·J Brahm
Sep 22, 1978·Biochimica Et Biophysica Acta·R I MaceyF W Orme
Feb 1, 1976·The Journal of General Physiology·M Dalmark
Jan 1, 1989·Annals of the New York Academy of Sciences·M L Jennings
Apr 1, 1989·Archives of Biochemistry and Biophysics·M Klingenberg
Jan 1, 1989·Annual Review of Biophysics and Biophysical Chemistry·M L Jennings
Jan 1, 1988·Annual Review of Physiology·P De WeerR F Rakowski
Sep 22, 1986·Biochimica Et Biophysica Acta·O Fröhlich, R B Gunn

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Citations

Dec 29, 2012·Pflügers Archiv : European journal of physiology·Fabian R ReimoldSeth L Alper
Jul 25, 1996·Biochimica Et Biophysica Acta·M V SerraC W Haest
Mar 1, 1997·The Journal of General Physiology·M N ChernovaS L Alper
Apr 1, 1999·Annual Review of Physiology·M F Romero, W F Boron
May 16, 2008·American Journal of Physiology. Cell Physiology·Marina N ChernovaSeth L Alper
Nov 26, 2011·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Fabian R ReimoldSeth L Alper
May 19, 2009·The Journal of Experimental Biology·Seth L Alper
Apr 20, 2007·Trends in Microbiology·Peter S SolomonRichard P Oliver
Apr 1, 2010·The New Phytologist·Jeremy J BougourePauline F Grierson
Oct 7, 2004·Fungal Genetics and Biology : FG & B·Roger R LewAshley Garrill
Sep 13, 2005·The Science of the Total Environment·Antonio BaezaJosé Luis Manjón
Aug 24, 2000·Fungal Genetics and Biology : FG & B·N DegouséeI B Heath
Apr 17, 2013·Physiological Reviews·Mark D Parker, Walter F Boron
Oct 22, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ryan M Pelis, J Larry Renfro
Jun 6, 2008·American Journal of Physiology. Renal Physiology·Stephen WalshHelene Guizouarn
Mar 11, 2008·Blood Cells, Molecules & Diseases·Seth L AlperCarlo Brugnara
Mar 6, 2002·Biochemistry·Hiroyuki KumaMichael L Jennings

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