Rapid embryonic cell cycles defer the establishment of heterochromatin by Eggless/SetDB1 in Drosophila

Genes & Development
C. A. SellerPatrick H O'Farrell

Abstract

Acquisition of chromatin modifications during embryogenesis distinguishes different regions of an initially naïve genome. In many organisms, repetitive DNA is packaged into constitutive heterochromatin that is marked by di/trimethylation of histone H3K9 and the associated protein HP1a. These modifications enforce the unique epigenetic properties of heterochromatin. However, in the early Drosophila melanogaster embryo, the heterochromatin lacks these modifications, which appear only later, when rapid embryonic cell cycles slow down at the midblastula transition (MBT). Here we focus on the initial steps restoring heterochromatic modifications in the embryo. We describe the JabbaTrap, a technique for inactivating maternally provided proteins in embryos. Using the JabbaTrap, we reveal a major requirement for the methyltransferase Eggless/SetDB1 in the establishment of heterochromatin. In contrast, other methyltransferases contribute minimally. Live imaging reveals that endogenous Eggless gradually accumulates on chromatin in interphase but then dissociates in mitosis, and its accumulation must restart in the next cell cycle. Cell cycle slowing as the embryo approaches the MBT permits increasing accumulation and action of Eggless at...Continue Reading

References

Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J C EissenbergS C Elgin
Jan 28, 1966·Science·S W Brown
Sep 7, 2002·Science·Ira M HallShiv I S Grewal
Dec 14, 2006·Development·Emily CloughTulle Hazelrigg
May 16, 2007·PLoS Genetics·Carole SeumSéverine Bontron
Jul 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Tsai-Yu TzengC-K James Shen
Oct 24, 2007·Molecular & Cellular Proteomics : MCP·Ulrich RothbauerHeinrich Leonhardt
Nov 28, 2007·Genetics·Carole SeumPierre Spierer
Feb 23, 2008·Current Biology : CB·Mark L McCleland, Patrick H O'Farrell
Mar 18, 2008·Nature Structural & Molecular Biology·Ke ZhangShiv I S Grewal
Oct 17, 2008·Molecular Cell·Hirohito HarukiUlrich K Laemmli
Aug 24, 2010·Insect Biochemistry and Molecular Biology·Matthew D RandTodd Clason
Nov 16, 2010·Current Biology : CB·Antony W ShermoenPatrick H O'Farrell
Aug 9, 2011·Current Biology : CB·Prashanth RanganRuth Lehmann
Mar 21, 2012·Genes & Development·Jeffrey A FarrellPatrick H O'Farrell
Oct 23, 2012·Current Biology : CB·Zhihuan LiMichael A Welte
Apr 17, 2013·Current Topics in Developmental Biology·Anas FadlounMaria-Elena Torres-Padilla
Aug 3, 2013·Cold Spring Harbor Perspectives in Biology·Sarah C R Elgin, Gunter Reuter
Feb 22, 2014·Trends in Genetics : TIG·Joel C Eissenberg, Sarah C R Elgin
Feb 22, 2014·Current Biology : CB·Kai YuanPatrick H O'Farrell
Sep 10, 2014·Annual Review of Genetics·Jeffrey A Farrell, Patrick H O'Farrell
Jan 20, 2015·Nature Methods·Jonathan B GrimmLuke D Lavis
Mar 21, 2015·Genes & Development·Constance AlabertAnja Groth
Apr 18, 2015·Biochimica Et Biophysica Acta·Michael A Welte
Oct 17, 2015·Science·Yang YuGregory J Hannon
Oct 24, 2015·Genes & Development·Grzegorz SienskiJulius Brennecke
Jun 25, 2016·Trends in Genetics : TIG·Kai YuanPatrick H O'Farrell
Aug 28, 2016·Genes & Development·Taylor J R PenkeRobert J Duronio

❮ Previous
Next ❯

Citations

Apr 3, 2019·Genes & Development·Robin L Armstrong, Robert J Duronio
Sep 29, 2019·FEBS Letters·Xiao HuShangqin Guo
Feb 1, 2020·Wiley Interdisciplinary Reviews. Developmental Biology·Chase C WesleyDaniel L Levy
Jul 3, 2019·Wiley Interdisciplinary Reviews. Developmental Biology·Gustavo AguilarShinya Matsuda
Sep 19, 2019·Proceedings of the National Academy of Sciences of the United States of America·Farah BughioKeith A Maggert
Feb 6, 2020·Annals of the New York Academy of Sciences·Jing LiuQi Zhou
Aug 14, 2020·Cells·Silvia Meyer-NavaViviana Valadez-Graham
Sep 4, 2019·Nature Reviews. Molecular Cell Biology·Claire MarchalDavid M Gilbert
Dec 9, 2020·Journal of Cell Science·Darya A KalashnikovaDmitry E Koryakov
Jan 10, 2021·International Journal of Molecular Sciences·Masamitsu YamaguchiHideki Yoshida
Jul 5, 2019·Bio-protocol·Stephen L McDaniel, Melissa M Harrison
Aug 23, 2021·Seminars in Cell & Developmental Biology·Jérémy Sallé, Nicolas Minc

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.