Rapid enzymatic detection of Escherichia coli contamination in polluted river water

Letters in Applied Microbiology
A H FarnleitnerR L Mach

Abstract

The relationship between the rate of beta-D-glucuronidase hydrolysis (GLUase-HR) and the E. coli concentration in rivers differing in the extent of faecal pollution was investigated. It was hypothesized that the rate of GLUase-HR is a better surrogate parameter for E. coli concentrations than estimated numbers of faecal coliforms (FC). The GLUase-HR of the water sample filter residues was determined as the rate of cleavage of 4-methylumbelliferyl-beta-D-glucuronide. FC and E. coli concentrations were enumerated using mFC and Chromocult Coliform agar, respectively. Regression analysis revealed that a 90% variation of the variable log GLUase-HR was directly related to the variable log E. coli concentrations. The observed relationship between the log of the FC count and the log of the GLUase activity could be explained by the hydrolysis activity of the E. coli population, as E. coli is a part of the FC group. The data suggest that the log of the GLUase-HR can be used as a surrogate parameter for the log of the E. coli concentrations. GLUase-HR determination may provide a rapid alternative technique to estimate E. coli concentrations in freshwaters.

References

Feb 1, 1989·Applied and Environmental Microbiology·G W ChangR Lum
Nov 1, 1994·Applied and Environmental Microbiology·C M DaviesJ L Stauber
May 1, 1994·Applied and Environmental Microbiology·L FiksdalI Midttun
Nov 1, 1993·Applied and Environmental Microbiology·K P BrennerA P Dufour
Jan 1, 1996·Trends in Microbiology·B D Preston, J P Dougherty
Apr 4, 2000·Journal of Applied Microbiology·I GeorgeP Servais
Oct 4, 2000·International Journal of Food Microbiology·M Manafi
Oct 6, 2000·Journal of Applied Microbiology·S O Van Poucke, H J Nelis

❮ Previous
Next ❯

Citations

Feb 22, 2011·Environmental Monitoring and Assessment·Nouho Koffi OuattaraPierre Servais
Jun 7, 2005·Marine Pollution Bulletin·Philippe LebaronP Servais
Sep 18, 2002·Journal of Applied Microbiology·G CarusoM Mancuso
Dec 9, 2010·International Journal of Environmental Research and Public Health·João P S Cabral
Jan 19, 2016·Enzyme and Microbial Technology·Nikou HesariMorteza Abbaszadegan
Mar 9, 2005·Letters in Applied Microbiology·T Garcia-ArmisenP Servais
May 20, 2008·Current Opinion in Biotechnology·Liv Fiksdal, Ingun Tryland
Feb 16, 2010·Water Research·Dirk WildeboerRamadan A Abuknesha
Dec 30, 2014·Ecotoxicology and Environmental Safety·Diana Mendes Silva, Lucília Domingues
Mar 14, 2009·Water Environment Research : a Research Publication of the Water Environment Federation·A Abusam, K J Keesman
Nov 9, 2019·Analytical and Bioanalytical Chemistry·Ciprian Briciu-BurghinaFiona Regan
Oct 27, 2020·Risk Analysis : an Official Publication of the Society for Risk Analysis·Émile SylvestreMichèle Prévost

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.