Rapid establishment of laboratory diagnostics for the novel coronavirus SARS-CoV-2 in Bavaria, Germany, February 2020

Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin
Regina KonradAndreas Sing

Abstract

The need for timely establishment of diagnostic assays arose when Germany was confronted with the first travel-associated outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in Europe. We describe our laboratory experiences during a large contact tracing investigation, comparing previously published real-time RT-PCR assays in different PCR systems and a commercial kit. We found that assay performance using the same primers and probes with different PCR systems varied and the commercial kit performed well.

References

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Citations

Apr 30, 2020·Journal of Medical Virology·Tianmin XuLonggen Liu
Apr 27, 2020·Digestive Endoscopy : Official Journal of the Japan Gastroenterological Endoscopy Society·P SinonquelR Bisschops
May 24, 2020·The FEBS Journal·Cassandra L AtzrodtThomas P Clements
Jun 26, 2020·The Cochrane Database of Systematic Reviews·Jonathan J DeeksUNKNOWN Cochrane COVID-19 Diagnostic Test Accuracy Group
Sep 10, 2020·Journal of Clinical Medicine·Giuseppe La Torre Covid-Sapienza Collaborative Group
Sep 29, 2020·Irish Journal of Medical Science·Grace Yixin ChanNiamh O'Flaherty
Jun 20, 2020·Journal of Medical Virology·Yang LiXuebing Yan
May 19, 2020·Archives of Pathology & Laboratory Medicine·Hui YangGuoping Xiong
Sep 24, 2020·Revista da Associação Médica Brasileira·Lincoln Lopes FerreiraWanderley Marques Bernardo
Aug 1, 2020·Journal of the American Society of Nephrology : JASN·Candice ClarkeMichelle Willicombe
Apr 23, 2020·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Daniel MathiesChristoph Bickel
Jun 25, 2020·Canadian Journal of Public Health = Revue Canadienne De Santé Publique·Igor BurstynPaul Gustafson
Jun 25, 2020·International Journal of Molecular Sciences·Tuna ToptanMarek Widera
Jun 26, 2020·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Maximilian MuenchhoffOliver T Keppler
Jun 4, 2020·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Max AugustinClara Lehmann
Aug 25, 2020·Critical Care Nursing Quarterly·Osakpolor OgbeborNitin Bhanot
Sep 19, 2020·Analytical and Bioanalytical Chemistry·Basant GiriBhanu B Neupane
Apr 16, 2020·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Anna S Fomsgaard, Maiken Worsøe Rosenstierne
Jun 4, 2020·Molecular Cancer·Wei FengShaoqing Ju
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Oct 8, 2020·Nature Reviews. Microbiology·Ben HuZheng-Li Shi
Oct 17, 2020·Critical Care Explorations·Claudia C Dos SantosJohn Marshall
Oct 29, 2020·Analytical Methods : Advancing Methods and Applications·Zhang ZhangGuoming Xie
Dec 16, 2020·PLoS Biology·Martin A M ReijnsAndrew P Jackson
Jan 5, 2021·Applied Microbiology and Biotechnology·Praveen RaiIddya Karunasagar
Jan 6, 2021·International Journal of Molecular Sciences·Valerie Bríd O'LearySaak Victor Ovsepian
Nov 2, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Lauren Hubert JaegerFrederico Pittella
Jan 17, 2021·Medical Microbiology and Immunology·Andreas OstermanOliver T Keppler
Jan 30, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Ute EberleUNKNOWN Bavarian SARS-CoV-2-Public Health Laboratory Team
Feb 5, 2021·Stroke; a Journal of Cerebral Circulation·Adnan I QureshiChi-Ren Shyu
Jan 27, 2021·European Journal of Clinical Investigation·Hagen Scherb
Nov 1, 2020·Journal of Infection and Public Health·Fahad AlsohimeSarah Al-Subaie
Nov 21, 2020·International Journal of Environmental Research and Public Health·Diego Fernández-LázaroJuan Mielgo-Ayuso

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Methods Mentioned

BETA
PCR

Software Mentioned

RealStar
Superscript
QuantiTect

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