Rapid estimation of numbers of fecal Bacteroidetes by use of a quantitative PCR assay for 16S rRNA genes

Applied and Environmental Microbiology
Linda K Dick, Katharine G Field

Abstract

Assessment of health risk associated with fecal pollution requires a reliable fecal indicator and a rapid quantification method. We report the development of a Taq nuclease assay for enumeration of 16S rRNA genes of Bacteroidetes. Sensitivity and correlation with standard fecal indicators provide experimental evidence for application of the assay in monitoring fecal pollution.

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Citations

Oct 31, 2006·Environmental Monitoring and Assessment·Tao Yan, Michael J Sadowsky
Dec 3, 2011·Environmental Science & Technology·Orin C ShanksRichard A Haugland
May 31, 2011·Applied and Environmental Microbiology·Jingrang LuNicholas Ashbolt
May 13, 2008·Applied and Environmental Microbiology·Jingrang LuStephen Hill
Jul 6, 2006·Applied and Environmental Microbiology·Sarah P Walters, Katharine G Field
Jan 14, 2009·Applied and Environmental Microbiology·Dennis L HansenRandall E Hicks
Feb 1, 2011·Applied and Environmental Microbiology·Christopher J Schulz, Gary W Childers
Jun 7, 2011·Applied and Environmental Microbiology·Shane W RogersOrin C Shanks
Feb 20, 2007·Applied and Environmental Microbiology·Donald M Stoeckel, Valerie J Harwood
Aug 4, 2006·Applied and Environmental Microbiology·Orin C ShanksKatharine G Field
Jun 1, 2010·Applied and Environmental Microbiology·Paul W J J van der Wielen, Gertjan Medema
Aug 2, 2011·Applied and Environmental Microbiology·Ryan J NewtonSandra L McLellan
Nov 15, 2011·Applied and Environmental Microbiology·Hyatt C GreenKatharine G Field
Apr 17, 2012·Applied and Environmental Microbiology·Catherine A KeltyOrin C Shanks
Feb 8, 2006·Applied and Environmental Microbiology·Rachel T NobleKenneth Schiff
Mar 7, 2006·Applied and Environmental Microbiology·Marilyn SouleDouglas R Call
May 10, 2007·Proceedings of the National Academy of Sciences of the United States of America·C D SinigallianoH M Solo-Gabriele
Oct 22, 2013·Applied and Environmental Microbiology·Adelumola OladeindeMarirosa Molina
Mar 6, 2009·Systematic and Applied Microbiology·Aaron M SaundersAndreas Schramm
Jul 24, 2007·Water Research·Katharine G Field, Mansour Samadpour
Sep 3, 2011·Environmental Microbiology·Hyatt C GreenKatharine G Field

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