Abstract
The development of therapeutic antibodies has grown over the last several years. Most of the recombinant monoclonal antibodies (mAbs) produced by mammalian cells are glycoproteins. Glycosylation of the mAbs can be associated with effector functions, such as antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity, as well as immunogenicity and clearance. Thus, mAb glycan heterogeneity is a significant characteristic associated with the safety and efficacy of the products. Therefore, glycan heterogeneity should be evaluated during research and development (R&D) and during development of mAbs manufacturing processes to identify the process parameters that affect glycan heterogeneity and to enhance understanding of the manufacturing process. There is an increasing need for a rapid, easy, and automated evaluation method for glycan heterogeneity. Liquid chromatography/mass spectrometry (LC/MS) is a method that can be used to analyze glycoforms. LC/MS is marked by the ability to measure the oligosaccharide composition of each glycoform, whereas other general methods, such as capillary electrophoresis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and ion-exchange chromatography, cannot. However, a l...Continue Reading
References
Jun 3, 2000·Biotechnology Progress·J P KunkelJ C Jamieson
May 18, 2001·Journal of Chromatography. a·H Z WanJ Cacia
Aug 28, 2001·Biochimie·U Galili
May 3, 2002·The Journal of Biological Chemistry·Robert L ShieldsLeonard G Presta
Jan 2, 2003·Nature Reviews. Drug Discovery·Ole Henrik Brekke, Inger Sandlie
Apr 22, 2004·Xenotransplantation·Yuko MiwaAkimasa Nakao
Sep 28, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Rinpei NiwaKenya Shitara
Feb 1, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Haleem J IssaqTimothy D Veenstra
Apr 20, 2006·Journal of Pharmaceutical and Biomedical Analysis·I ParisA Rieutord
Oct 13, 2006·Annual Review of Immunology·James N ArnoldRaymond A Dwek
Oct 13, 2006·Nature Biotechnology·Gary Walsh, Roy Jefferis
Jan 24, 2007·International Journal of Pharmaceutics·C BenedettiL Cartilier
Apr 6, 2007·AAPS PharmSciTech·Mohamed K GhorabArwinder Nagi
Feb 6, 2008·Analytical Biochemistry·Amareth LimBryan J Harmon
Jul 9, 2008·Current Opinion in Immunology·T Shantha Raju
Aug 2, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Elsa Wagner-RoussetAlain Beck
Aug 19, 2008·Journal of the American Society for Mass Spectrometry·Sandipan SinhaHimanshu S Gadgil
Dec 17, 2008·Current Pharmaceutical Biotechnology·Alain BeckNathalie Corvaïa
Jan 10, 2009·Nature Biotechnology·Anurag S Rathore, Helen Winkle
Jun 9, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ang LiuChad E Wujcik
Aug 8, 2009·International Journal of Pharmaceutics·Simin Hassannejad TabasiStephen W Hoag
Sep 12, 2009·Journal of the American Society for Mass Spectrometry·Carola W N DamenJos H Beijnen
May 12, 2010·MAbs·Hongwei XieJeffery R Mazzeo
May 19, 2010·Analytical and Bioanalytical Chemistry·A S RathoreV Ghare
May 19, 2010·Journal of Clinical Immunology·Robert M Anthony, Jeffrey V Ravetch
Sep 11, 2010·Nature Biotechnology·Gary Walsh
Nov 19, 2010·Bioanalysis·Katja HeinigAlmudena Gajate-Perez
Jul 16, 2011·Analytical Biochemistry·Timothy KaschakBoxu Yan
Citations
Nov 28, 2012·Trends in Biotechnology·Abhinav A Shukla, Uwe Gottschalk
Mar 10, 2015·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Kenichiro Todoroki
Jan 23, 2014·Biotechnology Progress·Kevin P BrowerJean Gamble
Aug 15, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yinan ChenYingyan Yu
May 28, 2013·Journal of Pharmaceutical and Biomedical Analysis·Akira HarazonoNana Kawasaki
Sep 3, 2013·MAbs·Sara RosatiAlbert J R Heck
Mar 17, 2015·Journal of Pharmaceutical and Biomedical Analysis·Hongbin LiuYonghua Taylor Zhang
Jun 22, 2016·Journal of Pharmaceutical and Biomedical Analysis·Maria Kristina ParrHouda Montacir
May 20, 2017·Rapid Communications in Mass Spectrometry : RCM·Kenichiro FurukiHideto Yamaguchi
Jan 23, 2019·MAbs·Christof ReglChristian G Huber
Feb 7, 2019·Critical Reviews in Biotechnology·André GuerraJarka Glassey
Mar 19, 2015·Biotechnology Letters·Jintao LiuXuping Liu
Nov 2, 2019·Journal of Pharmaceutical and Biomedical Analysis·Julien CamperiNathalie Delaunay
Nov 9, 2012·Analytical Chemistry·Alain BeckSarah Sanglier-Cianférani
May 2, 2014·Analytical Chemistry·Simone NicolardiYuri E M van der Burgt