Rapid evaluation of 25 key sphingolipids and phosphosphingolipids in human plasma by LC-MS/MS

Analytical and Bioanalytical Chemistry
Abdul BasitAndrea Armirotti

Abstract

We report on a new, sensitive, and fast LC-MS/MS method for the simultaneous determination of 25 key sphingolipid components in human plasma, including phosphorylated sphinganine and sphingosine, in a single 9-min run. This method enables an effective and high-throughput coverage of the metabolic changes involving the sphingolipidome during physiological or pathological states. The method is based on liquid-liquid extraction followed by reversed-phase LC-MS/MS. Exogenous odd-chain lipids are used as cost-effective but reliable internal standards. The method was fully validated in surrogate matrix and naive human plasma following FDA guidelines. Sample stability and dilution integrity were also tested and verified.

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Citations

Feb 24, 2016·Metabolomics : Official Journal of the Metabolomic Society·Abdul BasitAndrea Armirotti
Dec 1, 2015·Journal of Proteome Research·Bing Peng, Robert Ahrends
Aug 16, 2015·BMC Systems Biology·Weronika WronowskaAnna Gambin
Jun 28, 2016·Journal of Pharmaceutical and Biomedical Analysis·Rhishikesh ThakareYazen Alnouti
Jun 14, 2017·International Journal of Obesity : Journal of the International Association for the Study of Obesity·S HeinitzR Jumpertz von Schwartzenberg
May 10, 2018·Analytical and Bioanalytical Chemistry·Siddabasave Gowda B GowdaMakoto Arita
Nov 11, 2015·The Journal of Biological Chemistry·Natalia RealiniDaniele Piomelli
Aug 21, 2019·International Journal of Molecular Sciences·Valentina VozellaDaniele Piomelli
Jul 15, 2017·Scientific Reports·Alba Di PardoVittorio Maglione
Jan 10, 2018·Frontiers in Neuroscience·Alba Di PardoVittorio Maglione
Aug 12, 2017·Scientific Reports·Giovanna CapodiventoLucilla Nobbio
Jun 30, 2019·Analytical Biochemistry·Cosima Damiana CalvanoTommaso R I Cataldi
Apr 16, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Xuechen LiuHongliang Jiang
May 16, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Gwang Bin LeeMyeong Hee Moon

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Software Mentioned

LipidMaps
TargetLynx
MassLynx

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