Rapid evaluation of gonococcal and nongonococcal urethritis in men with Limulus amoebocyte lysate and a chromogenic substrate.

Journal of Clinical Microbiology
R B Prior, V A Spagna

Abstract

A chromogenic substrate was used with Limulus amoebocyte lysate (LAL) and compared by parallel testing with the traditional gelation LAL method for the rapid evaluation of exudative urethritis in 125 male patients. Of these patients, 67 had positive cultures for Neisseria gonorrhoeae and 58 were negative. The corresponding prevalence of gonococcal urethritis was 53.6%. For assay, diluted urethral samples and chromogenic substrate were added directly to single-test LAL vials, and objective color endpoint determinations were made visually after a 10-min incubation period at 37 degrees C. Sensitivity and specificity were 98.5% and 93.1%, respectively, with an overall accuracy in predicting culture results of 96.0%. The predictive value of a positive LAL test was 94.3% in our patient population; in a population with a prevalence of gonococcal urethritis of only 10%, the predictive value would be 61.3%. Results were not statistically different from those obtained by the 30-min gelation LAL method or by Gram-stained smears read by experienced microscopists (P greater than 0.05). Unlike the delicate gel, the color endpoint was not prone to accidental mechanical disruption during incubation or reading. Thus, use of a chromogenic substr...Continue Reading

References

Jan 1, 1975·Annals of Internal Medicine·N F Jacobs, S J Kraus
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Oct 15, 1980·Thrombosis Research·M F ScullyV V Kakkar

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Citations

Apr 19, 2006·Phytotherapy Research : PTR·Maki MotobuYoshikazu Hirota
Apr 1, 1995·Clinical Microbiology Reviews·J C Hurley
Feb 1, 1985·Journal of Clinical Microbiology·F N JudsonM R Shahan
May 1, 1985·Journal of Clinical Microbiology·R Nachum, R N Berzofsky

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