Rapid Evaluation of Spermidine from 12 Bean Cultivars by Direct Real-Time Mass Spectrometry Analysis

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Tao WuWeili Li

Abstract

The routine spermidine (SPD) detection method is time-consuming and laborious due to the lengthy chromatographic separation and/or tedious sample derivatization pretreatment. In this study, direct analysis in real-time ionization mode coupled with mass spectrometry (DART-MS) was developed to rapidly determine the SPD content of 12 bean cultivars. The results were compared in detail with those of the classical UHPLC-ESI-QTOF method. After conducting a series of optimizations, a simple sample extraction procedure employing 80% aqueous methanol, was followed by determination of sample extracts directly without any chromatographic separation or prior derivatization. The validated method showed excellent performance with low limits of detection (LOD of 0.025 mg·kg-1) and good recovery rates (102.79⁻148.44%). The investigation highlighted that the DART-MS method (~1.3 min per three samples) could be used as a high-throughput alternative to the classic UHPLC-ESI-QTOF method (~15 min per three samples).

References

Apr 15, 2005·Analytical Chemistry·Robert B CodyH Dupont Durst
Sep 15, 2005·Journal of Agricultural and Food Chemistry·M Beatriz A GlóriaM Selma Carvalho
Oct 6, 2009·Nature Cell Biology·Tobias EisenbergFrank Madeo
Oct 30, 2009·Analytical Chemistry·Daniel L G BorgesZoltán Mester
Feb 23, 2011·The Journal of Cell Biology·Eugenia MorselliGuido Kroemer
Mar 25, 2017·Mass Spectrometry·Li-Hua LiCheng-Chih Hsu
Jan 27, 2018·Science·Frank MadeoGuido Kroemer

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Methods Mentioned

BETA
electrophoresis
deamination

Software Mentioned

MS
DART

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