Rapid Extraction of DNA and rRNA from Sediments by a Novel Hydroxyapatite Spin-Column Method.

Applied and Environmental Microbiology
K J PurdyD B Nedwell

Abstract

We describe a novel hydroxyapatite spin-column method of nucleic acid extraction from natural sediments by which DNA and rRNA can be extracted separately. This very rapid method produces pure nucleic acid that can be utilized in some of the most common molecular biological procedures used in the analysis of natural microbial communities.

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Citations

Dec 17, 2002·The Journal of General and Applied Microbiology·Narito AsanumaTsuneo Hino
Nov 27, 2002·Applied and Environmental Microbiology·Tracy B NorrisTimothy R McDermott
Oct 9, 2003·Reviews on Environmental Health·Itziar AlkortaCarlos Garbisu
Nov 23, 2006·Applied Microbiology and Biotechnology·Zh H YangY Sh Liu
Sep 28, 2010·Applied and Environmental Microbiology·Yao PanPaul L E Bodelier
Dec 3, 2016·Nature Communications·Mark TrimmerKevin J Purdy
Jan 29, 2000·FEMS Microbiology Letters·J P BowmanT A McMeekin
Sep 14, 2007·FEMS Microbiology Ecology·Robert J Hawkins, Kevin J Purdy
Dec 10, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Christina WarinnerMatthew J Collins
May 25, 2007·FEMS Microbiology Letters·Ziv Arbeli, Cilia L Fuentes
Jan 20, 2006·FEMS Microbiology Ecology·Andrew BissettChris Burke
Jun 6, 2003·Applied and Environmental Microbiology·Gesche Braker, James M Tiedje
Jul 10, 2001·Letters in Applied Microbiology·M Orsini, V Romano-Spica
Jul 26, 2005·Electrophoresis·Changhyun RohRolf D Schmid
Apr 27, 2011·Applied Biochemistry and Biotechnology·Digvijay Verma, T Satyanarayana
Dec 15, 2012·World Journal of Microbiology & Biotechnology·Wei TianBiao Shen
Sep 26, 2001·Applied and Environmental Microbiology·R A HurtJ Zhou

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