Rapid field detection assays for Bacillus anthracis, Brucella spp., Francisella tularensis and Yersinia pestis

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
Pirjo MateroSimo Nikkari

Abstract

Rapid detection is essential for timely initiation of medical post-exposure prophylactic measures in the event of intentional release of biological threat agents. We compared real-time PCR assay performance between the Applied Biosystems 7300/7500 and the RAZOR instruments for specific detection of the causative agents of anthrax, brucellosis, tularemia and plague. Furthermore, an assay detecting Bacillus thuringiensis, a Bacillus anthracis surrogate, was developed for field-training purposes. Assay sensitivities for B. anthracis, Brucella spp., Francisella tularensis and Yersinia pestis were 10-100 fg of target DNA per reaction, and no significant difference in assay performance was observed between the instrument platforms. Specificity testing of the diagnostic panels with both instrument platforms did not reveal any cross-reactivity with other closely related bacteria. The duration of thermocycling with the RAZOR instrument was shorter, i.e. 40 min as compared with 100 min for the Applied Biosystems 7300/7500 instruments. These assays provide rapid tools for the specific detection of four biological threat agents. The detection assays, as well as the training assay for B. thuringiensis powder preparation analysis, may be uti...Continue Reading

References

Sep 9, 1999·The New England Journal of Medicine·T C DixonP C Hanna
Mar 19, 2002·Emerging Infectious Diseases·Simo NikkariDavid A Relman
Feb 8, 2003·Journal of Clinical Microbiology·Peter A EmanuelTed Hadfield
Feb 15, 2003·FEMS Microbiology Reviews·Manuel Porcar, Victor Juárez-Pérez
Jun 6, 2003·Biosensors & Bioelectronics·James A HigginsRonald P Koopman
Dec 10, 2003·Journal of Clinical Microbiology·Jessica L VersageJeannine M Petersen
Mar 9, 2004·Journal of Clinical Microbiology·William S ProbertMargot H Graves
Oct 9, 2004·Annual Review of Entomology·Kenneth L Gage, Michael Y Kosoy
Nov 18, 2004·Journal of Bacteriology·Fergus G PriestMartin C J Maiden
Aug 16, 2005·Clinical Chemistry·Catherine J ChaseDavid A Kulesh
May 18, 2006·Annual Review of Microbiology·Molly K McLendonLee-Ann H Allen
Feb 13, 2007·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·T SkottmanS Nikkari
Aug 2, 2007·Canadian Journal of Microbiology·G T Vilas-BôasO M N Arantes
Nov 1, 2007·Clinical Chemistry and Laboratory Medicine : CCLM·Sascha Al DahoukHerbert Tomaso
Jan 26, 2008·BMC Microbiology·Alain A Ocampo-Sosa, Juan M García-Lobo
Feb 10, 2009·Veterinary Microbiology·Lotfi BounaadjaBruno Garin-Bastuji
Sep 5, 2009·Diagnostic Microbiology and Infectious Disease·Katja A KoskelaSimo Nikkari

❮ Previous
Next ❯

Citations

Jan 25, 2013·Journal of Clinical Microbiology·Milena EulerManfred Weidmann
Apr 21, 2012·Journal of Clinical Microbiology·Milena EulerManfred Weidmann
Jan 26, 2013·Emerging Infectious Diseases·Ingmar JanseChantal Reusken
Mar 1, 2012·Journal of Virological Methods·Markos MölsäSimo Nikkari
Aug 16, 2016·Biologicals : Journal of the International Association of Biological Standardization·Sandra JulichHerbert Tomaso
Oct 26, 2016·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·Daniel SternLars Schaade
Aug 22, 2013·Current Opinion in Infectious Diseases·Matthew P RubachJohn A Crump
Nov 17, 2012·Expert Review of Molecular Diagnostics·Max Maurin
Mar 16, 2017·PLoS Neglected Tropical Diseases·Elisabeth Lindahl-RajalaUlf Magnusson
Jul 19, 2017·The American Journal of Tropical Medicine and Hygiene·Matthew L Robinson, Yukari C Manabe
Dec 13, 2019·MSphere·Kenneth B YehKay Mereish
Apr 25, 2021·Scientific Reports·James M AkokoCollins Ouma

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthrax Vaccines (ASM)

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.

Brucellosis

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.

Anthrax Vaccines

Three different types of anthrax vaccines are available; a live-attenuated, an alum-precipitated cell-free filtrate and a protein recombinant vaccine. The effectiveness between the three is uncertain, but the live-attenuated have shown to reduce the risk of anthrax with low adverse events. Here is the latest research on anthrax vaccines.

Anthrax

Anthrax toxin, comprising protective antigen, lethal factor, and oedema factor, is the major virulence factor of Bacillus anthracis, an agent that causes high mortality in humans and animals. Here is the latest research on Anthrax.

Brucellosis (ASM)

Brucellosis is a bacterial infection caused by members of the genus brucella and remains one of the world's major zoonotic diseases. Discover the latest research on Brucellosis here.