Rapid fluorescence-based measurement of toxicity in anaerobic digestion

Water Research
Jian Lin ChenDavid C Stuckey

Abstract

A rapid fluorescence measurement based on resazurin reduction was developed and applied for the detection of toxicants/inhibitors to anaerobic digestion metabolism. By initially using a pure facultative anaerobic strain, Enterococcus faecalis as a model organism, this technique proved to be fast and sensitive when detecting the model toxicant, pentachlorophenol (PCP). The technique revealed significant metabolic changes in Enterococcus faecalis with a PCP spike ranging from 0.05 to 100 mg/L, and could detect PCP's toxicity to E. faecalis at a concentration of only 0.05 mg/L in 8 min. Furthermore, by extending this technique to a mixed anaerobic sludge, not only could the effect of 0.05-100 mg/L PCP be determined on anaerobic digestion metabolism within 10 min, but also its rate of biogas production. These results suggest that a resazurin-based fluorescence measurement can potentially be incorporated into a microfluidic system to develop a biosensor for the real-time monitoring, control and early warning of toxicant/inhibitor loads in the influent to an anaerobic digestion system.

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Citations

Oct 13, 2015·Environmental Science & Technology·Jian Lin ChenDavid C Stuckey
Oct 17, 2017·Biotechnology and Bioengineering·Jian Lin ChenDavid C Stuckey
Feb 22, 2021·Journal of Microbiological Methods·Maritxu LabadieClaire-Emmanuelle Marcato-Romain
Jun 15, 2018·The Science of the Total Environment·Jian Lin ChenDavid C Stuckey

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