PMID: 11902919Mar 21, 2002Paper

Rapid fluorescence polarization immunoassay for the mycotoxin deoxynivalenol in wheat

Journal of Agricultural and Food Chemistry
C M Maragos, R D Plattner

Abstract

The fungus Fusarium graminearum, a pathogen of both wheat and maize, produces a toxin, deoxynivalenol (DON), that causes disease in livestock. A rapid test for DON in wheat was developed using the principle of fluorescence polarization (FP) immunoassay. The assay was based on the competition between DON and a novel DON-fluorescein tracer (DON-FL2) for a DON-specific monoclonal antibody in solution. The method, which is a substantial improvement over our previous DON FP immunoassay, combined a rapid (3 min) extraction step with a rapid (2 min) detection step. A series of naturally contaminated wheat and maize samples were analyzed by both FP immunoassay and liquid chromatography (HPLC-UV). For wheat the HPLC-UV and FP methods agreed well (linear regression r(2) = 0.936), but for maize the two methods did not (r (2) = 0.849). We conclude that the FP method is useful for screening wheat, but not maize, for DON.

References

May 1, 1996·Journal of Toxicology and Environmental Health·B A RotterJ J Pestka
Mar 23, 2001·Journal of Agricultural and Food Chemistry·C M MaragosM S Nasir

❮ Previous
Next ❯

Citations

Jun 1, 2006·Mycotoxin Research·C M Maragos
Feb 12, 2008·Analytical and Bioanalytical Chemistry·David S Smith, Sergei A Eremin
Oct 22, 2008·Analytical and Bioanalytical Chemistry·Rudolf Krska, Alexandra Molinelli
Apr 13, 2011·Analytical and Bioanalytical Chemistry·Denis DorokhinMichel W F Nielen
Sep 9, 2011·Analytical and Bioanalytical Chemistry·Vincenzo LippolisAngelo Visconti
Aug 1, 2011·Mycotoxin Research·Chris M Maragos
Aug 25, 2010·Journal of Agricultural and Food Chemistry·Julie MeneelyChristopher Elliott
Feb 16, 2010·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·C M Maragos, M Busman
Mar 31, 2009·International Journal of Molecular Sciences·Pietro CozziniChiara Dall'Asta
Jul 4, 2009·Journal of Chromatography. a·Zdenka Brenn-StruckhofovaEbrahim Razzazi-Fazeli
Nov 24, 2004·Luminescence : the Journal of Biological and Chemical Luminescence·Samira Sarter, Nadine Zakhia
Sep 3, 2004·Toxicology Letters·Elisabeth SchneiderErwin Märtlbauer
Apr 7, 2009·Analytica Chimica Acta·Hyang Sook ChunSergey A Eremin
May 20, 2008·Analytica Chimica Acta·Andrea C Galvis-SánchezIvonne Delgadillo
Jun 14, 2017·International Journal of Environmental Research and Public Health·Ahmad Alshannaq, Jae-Hyuk Yu
May 2, 2006·Mycopathologia·Michael Z ZhengJohann Binder
Jul 1, 2010·Prikladnaia biokhimiia i mikrobiologiia·A E UrusovB B Dzantiev
May 21, 2004·Journal of Food Protection·Chris M Maragos, Eun-Kyung Kim
Sep 11, 2017·EFSA Journal·UNKNOWN EFSA Panel on Contaminants in the Food Chain (CONTAM)Lutz Edler
Jan 1, 2010·Comprehensive Reviews in Food Science and Food Safety·Rajeev BhatA A Karim
Jul 28, 2009·Analytical Chemistry·Josephine RutaEric Peyrin
May 2, 2006·Environmental Science & Technology·Miriam M NgundiChris R Taitt

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.