Rapid, fluorometric DNA determination for chick limb-bud mesenchymal-cell microcultures

In Vitro Cellular & Developmental Biology. Animal
D F PaulsenD Adams

Abstract

Micromass cultures of chick and mouse limb-bud mesenchymal cells are commonly used for in vitro studies of cellular differentiation. Previously, adaptation of these cultures to 96-well plates facilitated analyses of various aspects of cellular behavior and the effects of different media components in these cultures. These adjustments allowed development of a serum-free medium for chick limb-bud mesenchymal cells and substantially decreased costs associated with media and reagents. Here we report a further development for this model system; a Hoechst 33342-based in situ DNA assay that provides reliable data much more quickly and with considerably less effort than had been feasible in the past. Because it allows quantitation of products of cellular differentiation and DNA in the same cultures, the number of cultures needed to provide the same data is essentially halved and the accuracy of normalized values for quantitative estimates of markers of differentiation is improved. Studies of the effects of retinoic acid on chick limb-bud mesenchymal cells were performed to document the usefulness of this method.

References

Nov 1, 1977·Analytical Biochemistry·J Kapuściński, B Skoczylas
Jan 15, 1979·Analytical Biochemistry·C F BrunkT W James
Oct 1, 1977·Developmental Biology·P B AhrensR S Reiter
Jan 1, 1992·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·H K PlummerC Y Dillard
Dec 1, 1988·Differentiation; Research in Biological Diversity·D F PaulsenM Solursh
Feb 1, 1988·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·D F Paulsen, M Solursh
Sep 6, 1972·Nature: New Biology·D Summerbell, L Wolpert
Apr 1, 1971·The Journal of Experimental Zoology·R A Finch, E Zwilling
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Nov 1, 1994·Anatomy and Embryology·D F Paulsen
Dec 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D F PaulsenB Johnson
May 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·D F PaulsenD Okello
Jan 1, 1996·Methods in Cell Biology·K DanielsM Solursh
Apr 1, 1964·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R LEV, S S SPICER
Mar 1, 1994·In Vitro Cellular & Developmental Biology. Animal·D F PaulsenW D Chen

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.