Rapid gas chromatographic profiling and screening of biologically active amines

Journal of Pharmaceutical and Biomedical Analysis
K R KimD H Jeong

Abstract

An efficient method is described for the simultaneous determination of 57 amines including volatile aliphatic amines, nonvolatile polyamines and catecholamines present in aqueous samples. The method is based on two-phase isobutyloxycarbonylation (isoBOC) with a pH shift. In 1.0 M phosphate buffer at pH 7.5, phenolic hydroxyl groups were allowed to react with isobutyl chloroformate in the dichloromethane phase, and subsequently pH of the aqueous phase was increased to 12.0 for the reaction of basic amino functions. The resulting N(O)-isoBOC amines were recovered by solid-phase extraction using Chromosorb P in normal phase partition mode, with subsequent tert.- butyldimethylsilylation of the remaining hydroxyl groups for gas chromatographic analysis. Using this combined procedure, linear responses were obtained in the concentration range of 0.2-12 ppm, with correlation coefficients varying from 0.945 to 0.999 for most of the amines studied except for 5-methoxytryptamine (0.864). Temperature-programmed retention index (I) sets as measured on DB-5 and DB-17 dual-capillary columns of different polarity were characteristic of each amine and thus, useful in the screening for amines by computer I matching. When applied to saliva sample...Continue Reading

References

Jan 26, 1990·Journal of Chromatography·H M Liebich, C Först
Mar 24, 1989·Journal of Chromatography·M J Avery, G A Junk
Jun 20, 1986·Journal of Chromatography·J F HollandC C Sweeley
Jan 1, 1987·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·S E ShephardW K Lutz
Dec 10, 1982·Journal of Chromatography·S YamamotoM Makita

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Citations

Jun 19, 2004·Archives of Pharmacal Research·Man-Jeong Paik, Kyoung-Rae Kim
Jul 12, 2002·Journal of Pharmaceutical and Biomedical Analysis·N A SantagatiG Ronsisvalle
Apr 6, 2004·Journal of Pharmaceutical and Biomedical Analysis·V GambaroG L Visconti
Nov 27, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Diana TetiHarold McNair
Jan 24, 2009·Clinica Chimica Acta; International Journal of Clinical Chemistry·Man Jeong PaikGwang Lee
Jan 25, 2007·Journal of Biochemistry and Molecular Biology·Man Jeong PaikGwang Lee
Jan 25, 2007·Journal of Biochemistry and Molecular Biology·Hiroaki NagaiHidenori Ichijo
Dec 3, 2016·Critical Reviews in Analytical Chemistry·Maciej MilanowskiBogusław Buszewski
May 12, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Guanshu LiuYinfa Ma
Jul 13, 2006·Journal of Separation Science·Veniero GambaroGiacomo L Visconti
Dec 5, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·A BouchereauF Larher

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