Rapid Gel Card Agglutination Assays for Serological Analysis Following SARS-CoV-2 Infection in Humans

ACS Sensors
Diana AlvesSimon R Corrie

Abstract

High-throughput and rapid serology assays to detect the antibody response specific to severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2) in human blood samples are urgently required to improve our understanding of the effects of COVID-19 across the world. Short-term applications include rapid case identification and contact tracing to limit viral spread, while population screening to determine the extent of viral infection across communities is a longer-term need. Assays developed to address these needs should match the ASSURED criteria. We have identified agglutination tests based on the commonly employed blood typing methods as a viable option. These blood typing tests are employed in hospitals worldwide, are high-throughput, fast (10-30 min), and automated in most cases. Herein, we describe the application of agglutination assays to SARS-CoV-2 serology testing by combining column agglutination testing with peptide-antibody bioconjugates, which facilitate red cell cross-linking only in the presence of plasma containing antibodies against SARS-CoV-2. This simple, rapid, and easily scalable approach has immediate application in SARS-CoV-2 serological testing and is a useful platform for assay development beyond the CO...Continue Reading

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Citations

Jan 15, 2021·Immunological Medicine·Norma Saad, Salim Moussa
Apr 1, 2021·Expert Review of Clinical Immunology·Elham MohitHossein Vahidi
Jul 9, 2021·Cell Reports Methods·Sally EsmailShawn S-C Li

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
electrophoresis
flow cytometry
Fluorescence
ELISA
ELISAs

Software Mentioned

GraphPad Prism
FlowJo

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