Rapid genome resequencing of an atoxigenic strain of Aspergillus carbonarius

Scientific Reports
Francisco Javier CabañesArmand Sánchez

Abstract

In microorganisms, Ion Torrent sequencing technology has been proved to be useful in whole-genome sequencing of bacterial genomes (5 Mbp). In our study, for the first time we used this technology to perform a resequencing approach in a whole fungal genome (36 Mbp), a non-ochratoxin A producing strain of Aspergillus carbonarius. Ochratoxin A (OTA) is a potent nephrotoxin which is found mainly in cereals and their products, but it also occurs in a variety of common foods and beverages. Due to the fact that this strain does not produce OTA, we focused some of the bioinformatics analyses in genes involved in OTA biosynthesis, using a reference genome of an OTA producing strain of the same species. This study revealed that in the atoxigenic strain there is a high accumulation of nonsense and missense mutations in several genes. Importantly, a two fold increase in gene mutation ratio was observed in PKS and NRPS encoding genes which are suggested to be involved in OTA biosynthesis.

References

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Datasets Mentioned

BETA
PRJEB6789

Methods Mentioned

BETA
PCR
chip
enzymatic shearing
electrophoresis

Software Mentioned

cnvnator
BLASTp
Blast2Go
SUPERW
REVIGO
SnpSift
AntiSmash
SNPeff
samtools
Clustal X

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