Rapid genotype "independent" Zea mays L. (maize) transformation via direct somatic embryogenesis

In Vitro Cellular & Developmental Biology. Plant : Journal of the Tissue Culture Association
Keith LoweWilliam Gordon-Kamm

Abstract

Constitutive expression of the Zea mays L. (maize) morphogenic transcription factors Baby Boom (Bbm) and Wuschel2 (Wus2) in maize can not only greatly increase transformation efficiency but can also induce phenotypic abnormalities and sterility. In an effort to alleviate the pleiotropic effects of constitutive expression, a genome wide search was undertaken to find suitable maize promoters to drive tissue and timing-specific expression of the transformation enhancing genes Bbm and Wus2. A promoter from a maize phospholipid transferase protein gene (Zm-PLTP pro ) was identified based on its expression in leaves, embryos, and callus while being downregulated in roots, meristems, and reproductive tissues. When Zm-PLTP pro driving Bbm was transformed into immature maize embryos along with a Wus2 expression cassette driven by the nopaline synthase promoter (Nos pro ::Wus2) abundant somatic embryos rapidly formed on the scutella. These embryos were individual and uniformly transformed and could be directly germinated into plants without a callus phase. Transformed plants could be sent to the greenhouse in as little as 1 mo and regenerated plants matched the seed-derived phenotype for the inbred and were fertile. However, T1 seed f...Continue Reading

References

Jun 1, 1997·The Plant Journal : for Cell and Molecular Biology·P K BuskM Pagès
Oct 3, 1999·Nature Biotechnology·M V MatzS A Lukyanov
Aug 13, 2002·The Plant Cell·Kim BoutilierMichiel M van Lookeren Campagne
Sep 17, 2002·Cell Death and Differentiation·L H FilonovaP V Bozhkov
Jun 13, 2006·BMC Bioinformatics·Koji KadotaKentaro Shimizu
Aug 15, 2006·Plant Physiology·Anuradha PujarSusan McCouch
Jun 23, 2007·Nature Protocols·Yuji IshidaToshihiko Komari
Aug 26, 1983·Science·B V CongerJ K McDaniel
Oct 30, 2013·The International Journal of Developmental Biology·Qing JiKan Wang
Jul 22, 2016·Genetics·Morgan E McCawJames A Birchler
Sep 8, 2016·The Plant Cell·Keith LoweWilliam Gordon-Kamm

❮ Previous
Next ❯

Citations

Nov 18, 2018·Plant Signaling & Behavior·Karina van der LindeVirginia Walbot
Aug 30, 2020·The Plant Journal : for Cell and Molecular Biology·Zongyou LvWansheng Chen
Mar 6, 2019·Annual Review of Plant Biology·Kunling ChenCaixia Gao
Jul 6, 2019·Frontiers in Plant Science·Taras Pasternak, Denes Dudits
Jan 28, 2020·Plant Cell Reports·Priyanka JhaVijay Kumar
Aug 22, 2020·Plant Biotechnology·Yuji IshidaToshihiko Komari
Dec 18, 2019·Nature Biotechnology·Michael F MaherDaniel F Voytas
Mar 11, 2020·Nature Biotechnology·Huirong GaoR Bob Meeley
Sep 29, 2020·Frontiers in Plant Science·Ning WangAjith Anand
Dec 12, 2018·Frontiers in Plant Science·William Patrick BewgChung-Jui Tsai
Oct 28, 2019·Communications Biology·Joshua K YoungRodolphe Barrangou
May 1, 2019·International Journal of Molecular Sciences·Brenda A López-RuizTzvetanka D Dinkova
Sep 26, 2020·Nature Reviews. Molecular Cell Biology·Haocheng ZhuCaixia Gao
Oct 20, 2018·Frontiers in Plant Science·Michael NagleSteven H Strauss
Oct 14, 2020·Nature Biotechnology·Juan M DebernardiJorge Dubcovsky
Jan 9, 2021·Current Opinion in Plant Biology·Ning Yang, Jianbing Yan
Dec 29, 2020·Journal of Integrative Plant Biology·Xiangqiang ZhanJose Ramon Botella
Dec 11, 2020·Nature Plants·Chris SchwartzSergei Svitashev
Mar 2, 2021·Transgenic Research·Teng-Kuei Huang, Holger Puchta
Jan 12, 2020·Communications Biology·Joshua K YoungRodolphe Barrangou
Mar 18, 2021·Transgenic Research·Rishikesh GhogareAmit Dhingra
Mar 3, 2019·Plant Science : an International Journal of Experimental Plant Biology·Albert P KauschZhanyuan J Zhang
Apr 24, 2021·Nature Communications·Zongliang ChenAndrea Gallavotti
Apr 20, 2021·Journal of Plant Physiology·Ravi B Anjanappa, Wilhelm Gruissem
Apr 27, 2021·Plant Communications·Shengchun LiJiang Zhang
May 4, 2021·Current Opinion in Plant Biology·Josh Strable, Hilde Nelissen
May 30, 2021·Proceedings of the National Academy of Sciences of the United States of America·Ryan A Nasti, Daniel F Voytas
Apr 7, 2021·The Plant Cell·Christophe GaillochetThomas B Jacobs
Jun 3, 2021·International Journal of Molecular Sciences·Mohsen HesamiAndrew Maxwell Phineas Jones
Aug 27, 2021·Annals of the New York Academy of Sciences·Jennifer CableRammyani Bagchi

❮ Previous
Next ❯

Datasets Mentioned

BETA
MF788073

Methods Mentioned

BETA
transgenic
RNAseq
light microscopy

Software Mentioned

DuPont Pioneer
Python
TIBCO Spotfire®

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.