Rapid green synthesis of silver nanoparticles from silver nitrate by a homeopathic mother tincture Phytolacca Decandra

Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine
Soumya Sundar BhattacharyyaAnisur Rahman Khuda-Bukhsh

Abstract

To examine if a homeopathic mother tincture (Phytolacca Decandra) is capable of precipitating silver nanoparticles from silver nitrate (AgNO(3)) and to characterize the biosynthesized nanoparticles for evaluating their biological activities. A total of 100 mg of AgNO(3) was added to 20mL of Milli-Q water and stirred vigorously. Then 5mL of the homeopathic mother tincture of Phytolacca Decandra (ethanolic root extract of Phytolacca decandra) was added and stirred continuously. Reduction took place rapidly at 300K and completed in 10 min as shown by stable light greenish-yellow color of the solution which gave colloid of silver nanoparticles. The colloid solution was then centrifuged at 5000×g to separate the nanoparticles for further use. The nanoparticles were characterized by spectroscopic analysis, particle size analysis and zeta potential measurements, and morphology was analyzed by atomic force microscopy. The drug-DNA interaction was determined by circular dichroism spectrophotometry and melting temperature profiles by using calf thymus DNA as the target. The biological activities were determined using a cancer cell line A549 in vitro and using bacteria Escherichia coli and fungus Saccharomyces cerevisiae as test models. P...Continue Reading

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Citations

Jan 11, 2014·Applied Microbiology and Biotechnology·Mahendra RaiMassimiliano Galdiero
Jan 8, 2013·Homeopathy : the Journal of the Faculty of Homeopathy·Iris R BellAudrey J Brooks
Apr 15, 2015·Homeopathy : the Journal of the Faculty of Homeopathy·Paolo BellaviteDebora Olioso
Jun 26, 2013·European Journal of Integrative Medicine·Iris R BellAudrey J Brooks
Jul 13, 2018·Artificial Cells, Nanomedicine, and Biotechnology·Krushna C HembramBirendra K Bindhani
Nov 25, 2020·Journal of Medicinal Chemistry·Mariana MoraisJoão A V Prior

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