Rapid heteromerization and phosphorylation of ligand-activated plant transmembrane receptors and their associated kinase BAK1.

The Journal of Biological Chemistry
Birgit SchulzeDelphine Chinchilla

Abstract

In plants leucine-rich repeat receptor kinases (LRR-RKs) located at the plasma membrane play a pivotal role in the perception of extracellular signals. For two of these LRR-RKs, the brassinosteroid receptor BRI1 and the flagellin receptor FLS2, interaction with the LRR receptor-like kinase BAK1 (BRI1-associated receptor kinase 1) was shown to be required for signal transduction. Here we report that FLS2.BAK1 heteromerization occurs almost instantaneously after perception of the ligand, the flagellin-derived peptide flg22. Flg22 can induce formation of a stable FLS2.BAK1 complex in microsomal membrane preparations in vitro, and the kinase inhibitor K-252a does not prevent complex formation. A kinase dead version of BAK1 associates with FLS2 in a flg22-dependent manner but does not restore responsiveness to flg22 in cells of bak1 plants, demonstrating that kinase activity of BAK1 is essential for FLS2 signaling. Furthermore, using in vivo phospholabeling, we are able to detect de novo phosphorylation of both FLS2 and BAK1 within 15 s of stimulation with flg22. Similarly, brassinolide induces BAK1 phosphorylation within seconds. Other triggers of plant defense, such as bacterial EF-Tu and the endogenous AtPep1 likewise induce rapi...Continue Reading

References

Jun 23, 1999·The Plant Journal : for Cell and Molecular Biology·G FelixT Boller
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·G FelixT Boller
Mar 5, 2002·Nature·Tsuneaki AsaiJen Sheen
Aug 2, 2002·Cell·Kyoung Hee Nam, Jianming Li
Jun 14, 2003·Plant Physiology·Shin Han Shiu, Anthony B Bleecker
Oct 14, 2003·Plant Physiology·Mark D Curtis, Ueli Grossniklaus
Apr 24, 2004·The Plant Cell·Shin-Han ShiuWen-Hsiung Li
Jan 21, 2006·PLoS Pathogens·Christopher Dardick, Pamela Ronald
Mar 3, 2006·Genes & Development·Silke RobatzekThomas Boller
Jun 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yube YamaguchiClarence A Ryan
Jun 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Alisa HuffakerClarence A Ryan
Feb 24, 2007·Molecular & Cellular Proteomics : MCP·Joris J BenschopFrank L H Menke
Jun 27, 2007·Cellular Microbiology·Clarence A RyanYube Yamaguchi
Jul 13, 2007·Nature·Delphine ChinchillaThomas Boller
Jul 13, 2007·Proceedings of the National Academy of Sciences of the United States of America·Antje HeeseJohn P Rathjen
Jul 27, 2007·The Plant Journal : for Cell and Molecular Biology·Thomas S NühseScott C Peck
Jan 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Man-Ho OhSteven C Huber
Dec 19, 2009·European Journal of Cell Biology·Sandra PostelThorsten Nürnberger

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Citations

May 11, 2013·Journal of Biosciences·Mehanathan Muthamilarasan, Manoj Prasad
Jun 30, 2010·Nature Reviews. Genetics·Peter N Dodds, John P Rathjen
Jul 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Freddy BoutrotJohn P Rathjen
Apr 6, 2011·Proceedings of the National Academy of Sciences of the United States of America·Yvon JaillaisJoanne Chory
May 10, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jianming Li
Nov 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Catherine AlbrechtCyril Zipfel
Nov 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Youssef BelkhadirJoanne Chory
Feb 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Zixu LiuJian-Min Zhou
Mar 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Cyril Zipfel
Aug 2, 2013·Molecular Plant-microbe Interactions : MPMI·Camilla Julie KørnerDagmar R Hann
Jan 5, 2011·Molecular Plant-microbe Interactions : MPMI·Shujing WuLibo Shan
Sep 28, 2012·Molecular Plant-microbe Interactions : MPMI·Brendan K RielyDouglas R Cook
Oct 6, 2010·Plant Physiology·Cyril Zipfel, Silke Robatzek
Jun 26, 2012·Plant Physiology·Alberto P MachoCyril Zipfel
Aug 28, 2012·The Plant Cell·Cécile SegonzacCyril Zipfel
Jun 2, 2012·Science·Tingting LiuJijie Chai
Jun 5, 2012·Yi chuan = Hereditas·Ya-Li ShiSan-Dui Guo
Sep 10, 2013·Journal of Experimental Botany·Markus Albert
Mar 13, 2012·Annual Review of Plant Biology·Benjamin Schwessinger, Pamela C Ronald
Aug 28, 2012·Annual Review of Phytopathology·Meritxell Antolín-LloveraMartin Parniske
Apr 12, 2014·Annals of Botany·Timo Engelsdorf, Thorsten Hamann
Dec 20, 2014·Cell Host & Microbe·Jacqueline MonaghanCyril Zipfel
Feb 18, 2016·Plant Molecular Biology·Ka-Wai Ma, Wenbo Ma
Feb 24, 2016·Frontiers in Physiology·Kelly M BalmantSixue Chen
Feb 20, 2016·The New Phytologist·Marcella A TeixeiraIsgouhi Kaloshian

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