Rapid, high efficiency isolation of pancreatic ß-cells

Scientific Reports
Susan M ClardyRalph Weissleder

Abstract

The ability to isolate pure pancreatic ß-cells would greatly aid multiple areas of diabetes research. We developed a fluorescent exendin-4-like neopeptide conjugate for the rapid purification and isolation of functional mouse pancreatic β-cells. By targeting the glucagon-like peptide-1 receptor with the fluorescent conjugate, β-cells could be quickly isolated by flow cytometry and were >99% insulin positive. These studies were confirmed by immunostaining, microscopy and gene expression profiling on isolated cells. Gene expression profiling studies of cytofluorometrically sorted β-cells from 4 and 12 week old NOD mice provided new insights into the genetic programs at play of different stages of type-1 diabetes development. The described isolation method should have broad applicability to the β-cell field.

References

Mar 22, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·B LukowiakF Pattou
Aug 27, 2005·Diabetes·Monica TetaJake A Kushner
Aug 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Qing LiHugh O McDevitt
Sep 30, 2010·Diabetes·Susan Bonner-WeirArun Sharma
Jul 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Thomas ReinerRalph Weissleder
Sep 2, 2011·Diabetologia·C DorrellM Grompe
Nov 28, 2013·Molecular and Cellular Endocrinology·Sarah Noerklit RoedMaria Waldhoer
Dec 18, 2013·Bioconjugate Chemistry·Susan M ClardyRalph Weissleder

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Citations

Jul 30, 2019·Journal of Food Biochemistry·Roberto Edén Laviada-CastilloVíctor Ermilo Arana-Argáez
Aug 30, 2020·Basic & Clinical Pharmacology & Toxicology·Ekramy ElmorsySoad Mosad
May 22, 2020·JCI Insight·Diana M ElizondoMichael W Lipscomb
Dec 16, 2020·Endocrine Reviews·Brent A McLeanDaniel J Drucker
Oct 11, 2020·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Ekramy ElmorsyJihan Ghulam
May 6, 2021·Metabolites·Nicholas NorrisMelkam Alamerew Kebede

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Methods Mentioned

BETA
flow cytometry
FCS

Software Mentioned

GenePattern
ImmGen

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