Rapid high-performance liquid chromatographic assay of dorzolamide in rabbit aqueous humor

Biomedical Chromatography : BMC
Adriana Maltese, Claudio Bucolo

Abstract

A rapid high-performance liquid chromatographic method using a C(18) reversed-phase column (Hypersil ODS) with UV detection at 254 nm and a simple pre-treatment of samples is presented for the analysis of dorzolamide, a carbonic anhydrase inhibitor, in rabbit aqueous humor. A water solution containing 2% ZnSO(4) small middle dot7H(2)O was used to deproteinize aqueous humor samples. The mobile phase consisted of 7% CH(3)CN and 93% of a solution containing 1% TEA adjusted to pH = 3.5 with H(3)PO(4). Paracetamol was found to be a suitable internal standard. The standard curves were linear in the detection range. The precision and the accuracy were <5% for both intra- and inter-day assays.

References

Jan 1, 1996·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·M F Sugrue
Jan 1, 1997·Drugs·J Clot
Dec 30, 1999·Progress in Retinal and Eye Research·M F Sugrue

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Citations

Mar 10, 2004·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Jun InoueMakoto Takehana
Apr 17, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Sonia T HassibRawda M Sayed
Jan 30, 2021·Drug Delivery·Sinar SayedOmnia Mohamed Khowessah

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