Rapid high performance liquid chromatographic assay for antifungal agents in human sera

The Journal of Antimicrobial Chemotherapy
T K NgA F Cheng

Abstract

Serum concentration of seven antifungal agents, amphotericin B, 5-flucytosine, ketoconazole, fluconazole, itraconazole, miconazole and econazole were assayed using a single step sample preparation and an isocratic High Performance Liquid Chromatography (HPLC) procedure based on three mobile phases of similar components. Our method was simple, flexible and rapid, the assays being completed within half an hour. The method showed high reproducibility, good sensitivity with detection limits of 0.078 to 0.625 mg/L except for miconazole and econazole, and high recovery rates of 86-l05%. Out of 24 therapeutic agents tested only aztreonam and trimethoprim were found to interfere with the assay of 5-flucytosine and fluconazole respectively, using this protocol. HPLC assay should be useful in the clinical laboratory for monitoring patients on antifungal therapy.

Citations

Jul 28, 2010·Archives of Pharmacal Research·Ibrahim A AlsarraSteven H Neau
Dec 4, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Jia Woei WongKah Hay Yuen
Mar 29, 2001·Journal of Pharmaceutical and Biomedical Analysis·T Eldem, N Arican-Cellat
Nov 4, 1998·Journal of Pharmaceutical and Biomedical Analysis·R Gage, D A Stopher
Feb 19, 2000·Antimicrobial Agents and Chemotherapy·S MachardE Ezan
Aug 22, 2000·Antimicrobial Agents and Chemotherapy·F BarchiesiG Scalise
Jul 18, 2009·Journal of Pharmaceutical and Biomedical Analysis·Jean-Baptiste GordienDominique Breilh
Dec 21, 2004·International Journal of Pharmaceutics·V PortaS Storpirtis
May 21, 2005·Therapeutic Drug Monitoring·Susan S BuchkowskyMary H H Ensom

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