Rapid, high-throughput extraction of bacterial genomic DNA from selective-enrichment culture media

Letters in Applied Microbiology
T Wilson, J Carson

Abstract

To create a fast, sensitive and inexpensive high-throughput method for the extraction of bacterial genomic DNA from selective-enrichment culture media. Lysis of bacteria was achieved using guanidinium isothiocyanate, and DNA was extracted using 96-well glass microfibre filtration plates. Extraction-PCR detected the presence of 1 cfu Yersinia ruckeri and 16 cfu Lactococcus garvieae 200 microl(-1) sample of selective-enrichment medium. An efficient method for high-throughput extraction of bacterial genomic DNA from selective-enrichment culture media was achieved. This method enables detection of covert bacterial infections in fish. The simultaneous extraction of large numbers of samples allows for its use in bacterial monitoring programmes and quarantine.

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Citations

Oct 16, 2003·Biosensors & Bioelectronics·Nathaniel C CadyCarl A Batt
Mar 30, 2011·Infection and Immunity·Yvonne Gar-Yun ChanJuan José Martinez
Jun 10, 2015·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Kan GaoHaifeng Wang
Jan 18, 2017·Applied Microbiology and Biotechnology·Xiaodong LiuGehong Wei
Jan 25, 2021·Environmental Geochemistry and Health·Anand Raj DhanapalPrabagaran Solai Ramatchandirane
Jul 10, 2016·Mitochondrial DNA. Part B, Resources·Yuan ZhangShaoxiong Ding
Dec 13, 2005·Journal of Chromatography. a·Lihua ZhangYoshinobu Baba

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