Rapid, highly sensitive gradient narrow-bore high-performance liquid chromatographic determination of suramin and its analogues

Journal of Chromatography. B, Biomedical Applications
M Kassack, P Nickel

Abstract

A high-performance liquid chromatography (HPLC) method for the determination of suramin, its precursors and analogues in aqueous solutions and in plasma samples with advantages compared to earlier methods is described. Due to the method's high sensitivity (detection limit of suramin in plasma samples: 7 ng/ml; in aqueous solutions: 5 ng/ml) and selectivity (suramin tR: 7.05 min, precursor amine 2 tR: 4.68 min), it is possible to analyze degradation products, impurities and possible metabolites of suramin besides suramin. Tetrabutylammonium hydrogensulfate (TBAHS) (5 mM) is used as ion-pairing reagent in a mixture of 36% methanol and 0.02 M phosphate buffer pH 6.5 is used as the mobile phase. After sample injection, a linear gradient from 36 to 62.9% methanol is run. A C8 stationary phase (100 x 2.1 mm I.D.) is used and ultraviolet (UV) detection at 238 nm is applied. Plasma extraction is performed with tetrabutylammonium bromide (pH 8.0) and acetonitrile. This procedure allows the determination of suramin and its precursor amine 2 in the range of 0.05-400 micrograms/ml with high precision [relative standard deviation of peak areas at 0.05 microgram/ml: 2.10% (n = 5)] and nearly complete recovery (> 96.5%). Because of the high f...Continue Reading

References

Sep 15, 1989·Biochemical Pharmacology·E L VansterkenburgL H Janssen
Mar 10, 1989·Deutsche medizinische Wochenschrift·B AllolioW Winkelmann
Oct 1, 1986·Biochemical Pharmacology·M D Coleman, K K Adjepon-Yamoah

❮ Previous
Next ❯

Citations

Jan 29, 2000·European Journal of Pharmacology·M KlapperstückF Markwardt
Jul 18, 2008·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M A Villalona-CaleroJ L-S Au
May 2, 2000·Anti-cancer Drugs·A Firsching-HauckB Allolio
Feb 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·M HoheneggerM Freissmuth
Jan 18, 2011·Analytica Chimica Acta·Andrew YuJoan M Esson
Aug 2, 2005·British Journal of Pharmacology·Ilse WolnerMartin Hohenegger
Apr 10, 2004·European Journal of Medicinal Chemistry·Matthias U KassackGünter Lambrecht
Jan 22, 2004·The Journal of Biological Chemistry·Daniel F McCainZhong-Yin Zhang
May 23, 2006·Pharmaceutical Research·Danny ChenJessie L-S Au
Oct 28, 2005·Journal of Medicinal Chemistry·Heiko UllmannMatthias U Kassack

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
E May, B Allolio
European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
K Holli, M Hakama
© 2021 Meta ULC. All rights reserved