Rapid HMB-45 staining in Mohs micrographic surgery for melanoma in situ and invasive melanoma

Journal of the American Academy of Dermatology
G M MenakerR L Moy

Abstract

Accurate interpretation of frozen sections in the treatment of melanoma by Mohs micrographic surgery may be difficult. Our purpose was to review the literature on the role of Mohs micrographic surgery in the treatment of melanoma and to demonstrate the added benefits of using rapid HMB-45 staining in Mohs micrographic surgery for the treatment of melanoma. Twenty cases of melanoma were included in our study. Histologic diagnosis in each case was made by means of excisional biopsy specimens and permanent sections. Mohs micrographic surgery was performed with 3-mm margins used for each stage. Each Mohs frozen section was stained with HMB-45. In addition, routine frozen sections stained with hematoxylin-eosin were also prepared for comparison. All tissues were also sent for permanent sections. These permanent sections were cut similarly to Mohs-oriented sections because they were sectioned horizontally. Since they were serving as the standard, no staining with HMB-45 was performed on these permanent sections. Further stages with 3-mm margins were taken until tissues stained negative. Frozen sections were compared with permanent sections at each stage of resection. Eleven of the 20 cases stained positive with HMB-45 antibody on the...Continue Reading

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Citations

Dec 21, 2004·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Daniel A DavisC William Hanke
Aug 14, 2002·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·John G AlbertiniMary F Farley
Oct 17, 2002·Dermatologic Clinics·John A Carucci
Nov 6, 2012·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·James S Trimble, Basil S Cherpelis
Jan 27, 2010·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Abdel Kader El TalDavid A Mehregan
Aug 19, 2006·Journal of Surgical Oncology·Claire L F Temple, John P Arlette
Nov 21, 2008·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Maya K ThosaniChih-Shan Jason Chen
Jul 21, 2005·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Daniel A DavisRobert A Robinson
Aug 3, 2011·Expert Review of Anticancer Therapy·Jeremy R EtzkornL Frank Glass
Jun 11, 2010·International Journal of Dermatology·Corinne Erickson, Stanley J Miller
Jul 8, 2011·Archives of Pathology & Laboratory Medicine·Jon A Reed, Christopher R Shea

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