Rapid hypoxia preconditioning protects cortical neurons from glutamate toxicity through delta-opioid receptor

Stroke; a Journal of Cerebral Circulation
Junhui ZhangYing Xia

Abstract

Hypoxia preconditioning (HPC), rapid or delayed, has been reported to induce neuroprotection against subsequent severe stress. Because delta-opioid receptor (DOR) plays an important role in delayed HPC-induced neuroprotection against severe hypoxic injury, we asked whether DOR is also involved in the rapid HPC-induced neuroprotection. Cultured rat cortical neurons at culture days 8 to 9 were exposed to a short-term hypoxia (1% O2 for 30 minutes) to induce HPC followed by 30-minute normoxia before exposing to glutamate toxicity (100 micromol/L; 4 hours). Neuronal viability was assessed by lactate dehydrogenase leakage and morphological assessment. Protein and mRNA levels of DOR were detected by receptor binding and RT-PCR, respectively. Naltrindole was used to block DOR. Developmental changes in NMDA receptor expression was measured by Western blots. HPC significantly reduced the glutamate-induced neuronal injury. Receptor binding showed that HPC increased DADLE (a DOR ligand) binding density in the cultured cortical neurons by >90% over control level (P<0.05), although RT-PCR did not detect any appreciable change in DOR mRNA. DOR inhibition with naltrindole had no effect on neuronal injury and completely abolished the HPC-induc...Continue Reading

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Citations

Sep 17, 2009·Cellular and Molecular Life Sciences : CMLS·Xuezhi KangYing Xia
Feb 4, 2009·Cellular and Molecular Life Sciences : CMLS·D ChaoY Xia
Jul 28, 2010·Canadian Journal of Anaesthesia = Journal Canadien D'anesthésie·Gregory M T Hare, Brian P Kavanagh
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Sep 28, 2012·Cellular and Molecular Life Sciences : CMLS·Xiaozhou HeYing Xia
Dec 29, 2009·Progress in Neurobiology·Dongman Chao, Ying Xia
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Feb 17, 2018·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Shahid Husain
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Dec 8, 2006·Acta neurochirurgica·H-J Steiger, D Hänggi
Apr 8, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Yue GengXuesong Tian

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