Rapid identification and antibiotic susceptibility testing of Yersinia pestis using bioluminescent reporter phage.

Journal of Microbiological Methods
David A SchofieldCaroline Westwater

Abstract

The rapid identification and antibiotic susceptibility testing of Yersinia pestis is paramount for a positive prognosis. We previously engineered a Y. pestis-specific 'bioluminescent' reporter phage for the identification of Y. pestis. In this study, we generated an improved reporter phage and evaluated the ability of this phage to provide direct and rapid susceptibility testing. Compared to the first generation reporter, the second generation reporter exhibited a 100-fold increase in signal strength, leading to a 10-fold increase in assay sensitivity. Y. pestis antimicrobial testing in the presence of the reporter elicited bioluminescent signals that were drug concentration-dependent, and produced susceptibility profiles that mirrored the standard CLSI method. The phage-generated susceptibility profiles, however, were obtained within hours in contrast to days with the conventional method.

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Oct 16, 2009·Journal of Clinical Microbiology·David A SchofieldCaroline Westwater
Apr 23, 2010·Emerging Infectious Diseases·David M WagnerPaul Keim

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Citations

Sep 20, 2012·Bioengineered·David SchofieldIan J Molineux
Sep 26, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·D A SchofieldI J Molineux
Jun 21, 2016·Journal of Applied Microbiology·P BárdyJ Doškař
Aug 8, 2021·Microorganisms·Shahar RotemRonit Aloni-Grinstein

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