Rapid identification and fingerprinting of Candida krusei by PCR-based amplification of the species-specific repetitive polymorphic sequence CKRS-1.

Journal of Clinical Microbiology
A CarlottiJ Villard

Abstract

A PCR method was developed to identify and fingerprint Candida krusei isolates simply and rapidly. The primer pair Arno1 and Arno2 was designed to amplify the polymorphic species-specific repetitive sequence CKRS-1 (C. krusei repeated sequence 1) that we identified in the nontranscribed intergenic regions (IGRs) of rRNA genes in C. krusei LMCK31. The specificity, sensitivity, reproducibility, and fingerprinting ability of the PCR assay were evaluated. Amplification products were obtained from all 131 C. krusei isolates studied. No other yeast species of medical importance (n = 26), including species similar to C. krusei, species of pathogenic filamentous fungi, or a variety of pathogenic bacteria, yielded a PCR product with these primers. This PCR assay allowed for the identification of C. krusei in less than 6 h. The PCR assay was sensitive enough to detect as little as 10 to 100 fg of C. krusei-purified DNA and proved to be reproducible. Since amplification products varied both in number and in molecular weight according to the strains, PCR patterns allowed strains to be distinguished. To ascertain the epidemiological usefulness of this PCR fingerprinting, the patterns of the 131 isolates were compared. A total of 95 types wh...Continue Reading

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Citations

Sep 16, 2011·Mycoses·Anita Hryncewicz-GwóźdźJacek C Szepietowski
Sep 2, 2015·Antimicrobial Agents and Chemotherapy·A ForastieroE Mellado
Jul 2, 2020·Infection and Drug Resistance·Manuela Gómez-Gaviria, Héctor M Mora-Montes
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Jul 16, 1999·Journal of Clinical Microbiology·F X HueC de Bievre
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Mar 4, 2000·Journal of Clinical Microbiology·J XuT G Mitchell
May 2, 2000·Journal of Clinical Microbiology·A K GuptaR C Summerbell
Nov 18, 2021·Journal of Clinical Microbiology·Merlijn H I van HarenJacques F Meis

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