Rapid identification and quantitative analysis of the chemical constituents in Scutellaria indica L. by UHPLC-QTOF-MS and UHPLC-MS/MS

Journal of Pharmaceutical and Biomedical Analysis
Lili HeJianhua Miao

Abstract

Ultra high performance liquid chromatography (UHPLC) coupled with a hybrid quadrupole time-of-flight mass spectrometry (QTOF-MS) was used to separate and identify the compounds in Scutellaria indica L. Fragmentation patterns of these compounds were investigated by the UHPLC-QTOF-MS technique in both negative and positive ion modes. By using this strategy, both intact precursors and fragment ions information were obtained from a single injection. A total of 42 phenolic and other compounds were unequivocally or tentatively identified from S. indica for the first time. Among them, 8 phenolic compounds: scutellarin, luteolin, naringenin, wogonoside, apigenin, hispidulin, wogonin and chrysin were further quantified and used as marker substances by multiple-reaction monitoring in negative ionization mode. The results demonstrated that the calibration curves for all analytes showed good linearity (R(2)>0.9992) within the test ranges. The overall limits of detection and limits of quantification were 0.12-10.52 ng/mL and 4.67-20.22 ng/mL, respectively. The recovery was 96.02-102.88% with a relative standard deviation of less than 3.02%. It is proposed that the methods described here can be applied for rapid evaluation, quality control a...Continue Reading

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Citations

Feb 22, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jingyuan XuMinjian Qin
Dec 2, 2018·Future Medicinal Chemistry·Da-Cheng HaoChang Liu
Oct 26, 2018·Rapid Communications in Mass Spectrometry : RCM·Rafał FrańskiMonika Beszterda
Jun 14, 2018·Journal of Chromatographic Science·Solomon K S Amoah, Maique W Biavatti
Jun 8, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ya-Chu FanChang-Yun Wang
Oct 12, 2018·The Journal of Pharmacy and Pharmacology·Gokhan ZenginMohamad Fawzi Mahomoodally
Jul 26, 2017·Journal of Chromatography. a·Hong WangZongtao Lin
Apr 21, 2021·Journal of Natural Medicines·Sho SanechikaKatsuya Ohbuchi

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