Rapid identification of Candida sp. by MALDI-TOF mass spectrometry subsequent to short-term incubation on a solid medium

APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica
Anne-Pauline BellangerLaurence Millon

Abstract

Rapid identification of Candida species is important for appropriate antifungal therapy of fungemia. The matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) system is a useful tool to identify bacteria and yeasts. In this study, we evaluated the feasibility of identifying yeasts after a short-term incubation on a solid medium. We tested 24 strains of eight Candida species. Blood culture bottles were spiked with a calibrated suspension of each Candida strain. Three different culture media, two types of blood culture bottles and three different incubation time points were tested. A multivariable random-effects logistic regression analysis was performed for determining factors independently associated with a successful MALDI-TOF MS identification. One-hundred and thirty-one out of 432 MALDI-TOF MS analyses (30%) exhibited a score ≥ 1.7. The performance of the technique varied across Candida species. Factors associated with a successful identification were the use of a chromogenic Candida medium and the time points 4 and 5 h. Using the factors 'chromogenic Candida medium' and time point 5 h the global performance of identification reached 60% and a mean MALDI-TOF score of 1.78. Identifying ye...Continue Reading

References

Jul 13, 2013·Journal of Microbiological Methods·Giovanna PulcranoMaria Rosaria Catania
Apr 5, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·E A IdelevichK Becker
Aug 7, 2014·Intensive Care Medicine·Olivier LortholaryUNKNOWN French Mycosis Study Group

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Citations

May 22, 2020·Clinical Proteomics·Fumio NomuraKazuyuki Matsushita
Jan 28, 2020·Journal of Microbiology and Biotechnology·Zhenbo XuBirthe V Kjellerup
Oct 30, 2020·Clinical Microbiology Reviews·Maryam Tidjani AlouDidier Raoult
Jul 3, 2021·Microorganisms·Marie-Gladys RobertDanièle Maubon

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