Rapid identification of cultured Mycobacterium tuberculosis with a panel of monoclonal antibodies in western blot and immunofluorescence.

Research in Microbiology
C P VerstijnenA H Kolk

Abstract

This paper describes the identification of cultured mycobacteria with a panel of monoclonal antibodies directed against species-specific epitopes in a Western blot (WB) test and in an immunofluorescence test (IFT). In WB, we identified mycobacteria of the Mycobacterium tuberculosis complex (M. tuberculosis, M. africanum, M. bovis, M. bovis BCG, and M. microti) with 10(8) bacteria. In the IFT, we identified mycobacteria of the M. tuberculosis complex, the M. avium complex (M. avium, M. intracellulare and M. scrofulaceum) and M. kansasii with 10(7) bacteria. Using a panel of 105 mycobacterial patient isolates, we compared identification by WB and IFT with conventional, culture and biochemical identification. Identification of the M. tuberculosis complex in WB had a specificity and sensitivity of 96.3 and 98.3%, respectively. Identification in IFT of the M. tuberculosis complex had a specificity and sensitivity of 94.6% and 89.7%, respectively. Since the identification of mycobacteria with mAb requires only a small number of bacteria, these tests will reduce by several weeks the time necessary for microbiological identification.

References

Mar 1, 1989·Reviews of Infectious Diseases·D B Young, A Mehlert
Jan 1, 1989·Research in Microbiology·D MoinardH Drugeon
May 1, 1988·The Journal of Infectious Diseases·L S Young
May 1, 1972·Canadian Journal of Microbiology·R Turcotte, Y Des Ormeaux
May 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·E StrausS Levine
Sep 1, 1981·Reviews of Infectious Diseases·M Tsukamura

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Citations

Dec 1, 1992·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·R G DeshpandeG Navalkar
Sep 15, 2010·Biomarker Insights·Rajpal S KashyapHatim F Daginawala
Sep 21, 2010·American Journal of Respiratory and Critical Care Medicine·Adithya CattamanchiJ Lucian Davis
Jan 15, 2004·Emerging Infectious Diseases·Alexandr D BotvinkinCharles E Rupprecht
Mar 20, 2003·Emerging Infectious Diseases·Yohko T AraiAlexandr D Botvinkin
Feb 10, 2019·Tropical Medicine and Infectious Disease·Rebecca ShipleyAshley C Banyard
Aug 1, 1991·Journal of Clinical Microbiology·J W FriesD F Wirth

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