Rapid identification of cytochrome P450 inductive constituents of Shenmai injection by combined pregnane X receptor reporter gene assay and LC-TOF-MS analysis.

Journal of Ethnopharmacology
Zhen-Wei YuLe-Le Ge

Abstract

Identifying the inductive constituents of cytochrome P450 (CYP) enzymes is important in characterizing the safety of ethnopharmacological herbal preparations. This study provides a rapid and accurate method for screening CYP inducers in Shenmai injection (SMI), a traditional Chinese medicine. We combined a pregnane X receptor (PXR) reporter gene assay and liquid chromatography time-of-flight mass spectrometry (LC-TOF-MS) analysis to screen ethanol and aqueous extracts of SMI for CYP-inducing constituents. The ethanol extract exhibited stronger PXR activity than the aqueous extract. Of the 29 chemical compounds identified, 7 compounds with high relative concentrations in the ethanol extract were further evaluated for PXR activity. The highest activity was exhibited by methyl ophiopogonanone B and ginsenoside F2, indicating that they are CYP inducers. The identification method applied in this study was rapid and accurate and is suitable for screening CYP inducers in herbal preparations.

References

Sep 23, 2008·Chemico-biological Interactions·Chunna YuSu Zeng
Apr 29, 2011·Journal of Ethnopharmacology·Chunna YuSu Zeng
Nov 20, 2016·European Journal of Drug Metabolism and Pharmacokinetics·Meenakshi R Ramanathan, Scott R Penzak
Jan 27, 2017·Current Drug Targets·Palrasu Manikandan, Siddavaram Nagini
Oct 29, 2017·Archives of Pharmacal Research·Jee Sun Min, Soo Kyung Bae
Jan 25, 2018·British Journal of Clinical Pharmacology·Charles AwortweBernd Rosenkranz
Apr 29, 2018·Journal of Food and Drug Analysis·Brett C HogleWen Xie
Mar 28, 2019·Current Drug Metabolism·Mohammad K Parvez, Vikas Rishi

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Citations

Apr 27, 2021·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Han LiYue Gao

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