Rapid identification of Enterobacteriaceae by sequencing DNA gyrase subunit B encoding gene

Diagnostic Microbiology and Infectious Disease
Julien DelmasMonique Chomarat

Abstract

Real-time polymerase chain reaction and sequencing were used to characterize a 506-bp-long DNA fragment internal to the gyrB gene (gyrBint). The sequences obtained from 32 Enterobacteriaceae-type strains and those available in the Genbank nucleotide sequence database (n = 24) were used as a database to identify 240 clinical enterobacteria isolates. Sequence analysis of the gyrBint fragment of 240 strains showed that gyrBint constitutes a discriminative target sequence to differentiate between Enterobacteriaceae species. Comparison of these identifications with those obtained by phenotypic methods (Vitek 1 system and/or Rapid ID 32E; bioMérieux, Marcy l'Etoile, France) revealed discrepancies essentially with genera Citrobacter and Enterobacter. Most of the strains identified as Enterobacter cloacae by phenotypic methods were identified as Enterobacter hormaechei strains by gyrBint sequencing. The direct sequencing of gyrBint would be useful as a complementary tool in the identification of clinical Enterobacteriaceae isolates.

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Citations

Mar 8, 2008·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·X-L HouZ Chen
Jan 19, 2008·Journal of Biosciences·B R Mohanty, P K Sahoo
Jun 12, 2009·Journal of Clinical Microbiology·Philippe C MorandValérie Dumaine
Sep 28, 2007·Canadian Journal of Microbiology·Daniela Santos PontesAndréa Maria Amaral Nascimento
Mar 21, 2007·Diagnostic Microbiology and Infectious Disease·Hong Nhung PhamTakayuki Ezaki
Nov 27, 2015·BMC Bioinformatics·Dhany SaputraThomas Sicheritz-Pontén
Aug 3, 2013·International Journal of Systematic and Evolutionary Microbiology·Roberto A SouzaJuliana P Falcão
Jul 23, 2014·Microbes and Infection·Sharon Yvette Angelina M VillanuevaShin-ichi Yoshida
Jan 17, 2017·Scientific Reports·Sana HamdiSylvain Moineau
Nov 4, 2017·Viruses·Julia VillarroelMorten Nielsen
Nov 11, 2017·International Journal of Systematic and Evolutionary Microbiology·Marcelo PillonettoFabio Rezzonico
Jun 7, 2020·Applied and Environmental Microbiology·Jocelin RizoRomina Rodríguez-Sanoja
Jun 15, 2018·Journal of Clinical Microbiology·Christine MartineauCaroline Quach
Feb 29, 2020·Infection and Drug Resistance·Jian-Jun GouBei-Wen Zheng
May 27, 2016·Letters in Applied Microbiology·Carrie BradyDawn Arnold
Apr 4, 2021·Microorganisms·Valerio BaldelliFederica Del Chierico

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