Rapid Identification of KL49 Acinetobacter baumannii Associated with Clinical Mortality

Infection and Drug Resistance
Qiuyang DengWei Huang

Abstract

We aimed to establish a tool for rapid identification of KL49 Acinetobacter baumannii. Based on the capsular polysaccharide (CPS) synthesis genes database, we investigated the distribution of K locus type 49 (KL49) genes in other KL types and established a rapid identification method for KL49. We collected 61 clinical carbapenem-resistant A. baumannii (CRAB) strains, identified KL49 by gtr100 detection, and used whole genome sequencing (WGS) for verification. A mouse pneumonia model was used to confirm the hypervirulence phenotype. We tested the presence of gtr100 gene in 165 CRAB strains from three provinces in China and evaluated the correlation of gtr100 carrying CRAB infection with mortality. The gtr100 gene is the CPS synthesis gene found only in KL49. We screened out nine WGS-validated KL49 strains from 61 CRAB clinical strains using polymerase chain reaction (PCR) to detect the gtr100 gene. The survival rates of KL49 strains were significantly lower than nonKL49 strains in a mouse pneumonia model. The survival rates of LAC-4 gtr100 knockout strain decreased significantly. Analysis of phylogenetics showed the worldwide spread of KL49 A. baumannii. Infection of gtr100 carrying CRAB is an independent risk for mortality (OR,...Continue Reading

References

Oct 8, 2005·American Journal of Respiratory and Critical Care Medicine·Sylvia KnappTom van der Poll
Jun 30, 2007·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Chi-Tai FangShan-Chwen Chang
Nov 17, 2007·Nature Reviews. Microbiology·Lenie DijkshoornHarald Seifert
Jul 21, 2010·Infection and Immunity·Thomas A RussoAnthony A Campagnari
May 22, 2013·Antimicrobial Agents and Chemotherapy·Greg HarrisWangxue Chen
Apr 3, 2014·Carbohydrate Research·Evgeny VinogradovWangxue Chen
Apr 1, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Crystal L JonesEmil Lesho
Nov 22, 2015·Bioinformatics·Dmitry AntipovPavel A Pevzner
Nov 14, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kai ZhouChao Zhuo
Nov 14, 2018·Bioinformatics·Shifu ChenJia Gu
Dec 26, 2018·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·X ZengY Shi
Apr 2, 2019·Nucleic Acids Research·Ivica Letunic, Peer Bork

❮ Previous
Next ❯

Methods Mentioned

BETA
glycosylation
PCR

Software Mentioned

Parsnp
fastp
RAxML
SPAdes Genome Assembler
iTOL
Kaptive
ABRicate

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology
O HenigR D Balicer
Journal of the Medical Association of Thailand = Chotmaihet Thangphaet
Uraporn PhumisantiphongKanokrat Siripanichgon
American Journal of Infection Control
Iara RossiRosineide Marques Ribas
© 2021 Meta ULC. All rights reserved