Rapid identification of lectin receptors and their possible function in sea urchin cell systems

Acta histochemica
V H LathamSteven B Oppenheimer

Abstract

An assay using lectin derivatized agarose beads to rapidly and inexpensively identify cell surface lectin receptors was recently described by Latham et al. (1995). In this earlier study, the assay was tested on large, early stage sea urchin embryo cells. In this study this assay was used to examine lectin receptors on small, later stage sea urchin embryo cells that are more typical of cells that most investigators deal with, to ascertain if cell size is a determining factor in the assay's validity. The results indicated that the assay is a valid method to identify lectin receptors on small as well as large cells. Twenty-three hour Strongylocentrotus purpuratus embryo cells strongly bound Triticum vulgaris, concanavalin A, Artocarpus integrifolia and Vicia villosa using both the agarose bead and fluorescence assays, while three other lectins, Ulex europaeus I, Lotus tetragonolobus and Lens culinaris did not strongly bind to the cells using these two assays. As in earlier studies agglutinability results did not correlate well with results using the two other assays. In all cases where lectin bead binding, fluorescent lectin binding or lectin-mediated agglutination occurred, specific sugars reduced the observed binding. The second...Continue Reading

References

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Aug 1, 1994·Zygote : the Biology of Gametes and Early Embryos·M F DailyE G Pollock
Aug 29, 1986·Science·E Spiegel

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Citations

Jan 16, 1998·Acta histochemica·J PhilipS B Oppenheimer
May 20, 1998·Acta histochemica·V H LathamS B Oppenheimer
Aug 12, 1999·Acta histochemica·V H Latham, S B Oppenheimer
Aug 12, 1999·Acta histochemica·B A SalbillaS B Oppenheimer
May 8, 2002·Acta histochemica·Vanessa M NavarroSteven B Oppenheimer
Oct 23, 2002·Acta histochemica·Maria R KhurrumSteven B Oppenheimer
May 19, 2004·Acta histochemica·Maria KhurrumSteven B Oppenheimer
Sep 27, 2005·Acta histochemica·Cathy Coyle-Thompson, Steven B Oppenheimer
Sep 27, 2005·Acta histochemica·Eileen L HeinrichSteven B Oppenheimer

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