Rapid identification of Legionella species by mass spectrometry

Journal of Medical Microbiology
Claire MolinerPierre-Edouard Fournier

Abstract

Legionella species are facultative, intracellular bacteria that infect macrophages and protozoa, with the latter acting as transmission vectors to humans. These fastidious bacteria mostly cause pulmonary tract infections and are routinely identified by various molecular methods, mainly PCR targeting the mip gene and sequencing, which are expensive and time-consuming. Recently, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has emerged as a rapid and inexpensive method for identification of bacterial species. This study evaluated the use of MALDI-TOF-MS for rapid species and serogroup identification of 21 Legionella species recognized as human pathogens. To this end, a reference MS database was developed including 59 Legionella type strains, and a blind test was performed using 237 strains from various species. Two hundred and twenty-three of the 237 strains (94.1 %) were correctly identified at the species level, although ten (4.2 %) were identified with a score lower than 2.0. Fourteen strains (5.9 %) from eight species were misidentified at the species level, including seven (3.0 %) with a significant score, suggesting an intraspecific variability of protein profiles within some sp...Continue Reading

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Citations

Apr 2, 2010·Journal of Clinical Microbiology·Marc O SiegelSteven M Holland
Jan 14, 2011·Journal of Clinical Microbiology·Marc Roger CouturierMark A Fisher
Aug 28, 2010·BMC Bioinformatics·Ali Al-ShahibSaheer Gharbia
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Jul 1, 2015·Analytical and Bioanalytical Chemistry·Dragana KusićJürgen Popp
Dec 11, 2013·Diagnostic Microbiology and Infectious Disease·John A BrandaMary Jane Ferraro
Oct 10, 2013·Water Research·Dragana KusićJürgen Popp
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