Rapid identification of nitrogen-fixing and legume-nodulating Burkholderia species based on PCR 16S rRNA species-specific oligonucleotides

Systematic and Applied Microbiology
Arnoldo Wong-Villarreal, J Caballero-Mellado

Abstract

Several novel N(2)-fixing Burkholderia species associated with plants, including legume-nodulating species, have recently been discovered. Presently, considerable interest exists in studying the diazotrophic Burkholderia species, both for their ecology and their great potential for agro-biotechnological applications. However, the available methods used in the identification of these Burkholderia species are time-consuming and expensive. In this study, PCR species-specific primers based on the 16S rRNA gene were designed, which allowed rapid, easy, and correct identification of most known N(2)-fixing Burkholderia. With this approach, type and reference strains of Burkholderia kururiensis, B. unamae, B. xenovorans, B. tropica, and B. silvatlantica, as well as the legume-nodulating B. phymatum, B. tuberum, B. mimosarum, and B. nodosa, were unambiguously identified. In addition, the PCR species-specific primers allowed the diversity of the diazotrophic Burkholderia associated with field-grown tomato and sorghum plants to be determined. B. tropica and B. xenovorans were the predominant species found in association with tomato, but the occurrence of B. tropica with sorghum plants was practically exclusive. The efficiency of the speci...Continue Reading

References

Mar 21, 2001·FEMS Microbiology Letters·J RichardsonR H Coutts
May 26, 2001·Applied and Environmental Microbiology·P Estrada-De Los SantosJ Caballero-Mellado
Dec 26, 2001·Bioinformatics·S KumarM Nei
Mar 28, 2002·Applied and Environmental Microbiology·Joana Falcão SallesJan Dirk van Elsas
Jul 31, 2002·Applied and Environmental Microbiology·Suzanne C M MillerJennifer L Parke
Jul 29, 2004·International Journal of Systematic and Evolutionary Microbiology·Jesús Caballero-MelladoPaulina Estrada-De los Santos
Nov 17, 2004·International Journal of Systematic and Evolutionary Microbiology·V M ReisJ Caballero-Mellado
Jan 11, 2005·Applied and Environmental Microbiology·María ValdésAnn M Hirsch
Jan 12, 2005·Nature Reviews. Microbiology·Eshwar MahenthiralingamJoanna B Goldberg
Feb 16, 2005·Systematic and Applied Microbiology·Craig F Barrett, Matthew A Parker
Mar 5, 2005·Applied and Environmental Microbiology·Alban RametteJames M Tiedje
Jun 18, 2005·Letters in Applied Microbiology·L A O'Sullivan, E Mahenthiralingam
Jul 8, 2005·Applied and Environmental Microbiology·George W PayneEshwar Mahenthiralingam
Feb 2, 2006·International Journal of Systematic and Evolutionary Microbiology·Hee-Chan YangSung-Taik Lee
Feb 8, 2006·Applied and Environmental Microbiology·Craig F Barrett, Matthew A Parker
May 5, 2006·Applied and Environmental Microbiology·L PerinJ Caballero-Mellado
Aug 12, 2006·International Journal of Systematic and Evolutionary Microbiology·Wen-Ming ChenPeter Vandamme
Aug 12, 2006·International Journal of Systematic and Evolutionary Microbiology·L PerinJ Caballero-Mellado
Oct 3, 2006·International Journal of Systematic and Evolutionary Microbiology·Angel ValverdeJosé M Igual
May 3, 2007·International Journal of Systematic and Evolutionary Microbiology·Wen-Ming ChenPeter Vandamme
Jul 3, 2007·Applied and Environmental Microbiology·Jesús Caballero-MelladoLourdes Martínez-Aguilar
Apr 22, 2008·FEMS Microbiology Reviews·Stéphane CompantEssaïd Ait Barka
May 27, 2008·Applied and Environmental Microbiology·Lourdes Martínez-AguilarJesús Caballero-Mellado
Jun 2, 2009·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Zakuan Zainy DerisManicham Ravichandran

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Citations

May 18, 2010·Applied and Environmental Microbiology·C TalbiE J Bedmar
Aug 19, 2011·Microbial Ecology·Zulma Rocío Suárez-MorenoVittorio Venturi
Aug 13, 2011·Molecular Plant-microbe Interactions : MPMI·Prasad GyaneshwarEuan K James
Feb 14, 2013·Current Microbiology·Paulina Estrada-de los SantosJesús Caballero-Mellado
May 29, 2018·World Journal of Microbiology & Biotechnology·Pamela Romina BernabeuMaría Flavia Luna

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