Rapid identification of pathogenic Leptospira species (Leptospira interrogans, L. borgpetersenii, and L. kirschneri) with species-specific DNA probes produced by arbitrarily primed PCR.

Journal of Clinical Microbiology
M LetocartP Perolat

Abstract

Arbitrarily primed PCR (AP-PCR) assays can be used to discriminate between species of Leptospira. Comparative analysis of the fingerprints obtained from representative sets of serovar reference strains of Leptospira interrogans sensu stricto, L. borgpetersenii, and L. kirschneri and the reference strains of the other Leptospira spp. revealed species-specific DNA fragments. These species-specific sequences were reamplified in order to produce digoxigenin-11-dUTP-labeled genomic DNA probes that could be used to identify Leptospira species. Three probes (specific for L. interrogans sensu stricto, L. borgpetersenii, and L. kirschneri) were selected and tested with 72 representative serovar reference strains, all of which had previously been studied by DNA-DNA hybridization. The two techniques were in general agreement, and hybridization with AP-PCR-derived probes was shown to be a useful approach for rapid species determination of leptospires, without the prior need for DNA sequence information. These nonradioactive probes can be used to identify Leptospira species in nonspecialized laboratories, and this should contribute to a better knowledge of the molecular epidemiology of leptospirosis.

References

Apr 1, 1992·International Journal of Systematic Bacteriology·P RamadassR B Marshall
Mar 1, 1990·Journal of Clinical Microbiology·R BoomJ van der Noordaa
Nov 25, 1990·Nucleic Acids Research·J G WilliamsS V Tingey
Feb 1, 1990·Research in Microbiology·P PérolatG Baranton
Dec 25, 1990·Nucleic Acids Research·J Welsh, M McClelland
Mar 1, 1988·Applied and Environmental Microbiology·L V Stamm, N W Charon
Jun 1, 1982·Journal of Clinical Microbiology·D J BrennerA G Steigerwalt
Dec 1, 1995·Journal of Clinical Microbiology·R L ZuernerC A Bolin

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Citations

Apr 9, 2001·Clinical Microbiology Reviews·P N Levett
May 13, 2014·International Journal of Environmental Research and Public Health·Ahmed AhmedRudy A Hartskeerl
Sep 2, 2011·Journal of Clinical Microbiology·E C RomeroR L Galloway
Dec 1, 2020·PloS One·Elise Klement-FrutosCyrille Goarant
Mar 2, 1999·Applied and Environmental Microbiology·C GoarantP Perolat

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