PMID: 9189139Jun 1, 1997Paper

Rapid imaging of experimental infection with technetium-99m-DTPA after anti-DTPA monoclonal antibody priming

Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine
M H KranenborgO C Boerman

Abstract

Antibodies accumulate nonspecifically in infectious foci due to the locally increased vascular permeability. This study describes a method of infection imaging in which 99mTc-DTPA (diethylenetriaminepentaacetic acid) is trapped at the target by a previously administered anti-DTPA monoclonal antibody. DTIn1. Rats with Staphylococcus aureus-infected calf muscle were injected intravenously with DTIn1. Two to 24 hr after the DTIn1 injection, 99mTc-DTPA was injected intravenously. In separate experiments, excess DTIn1 was cleared from the circulation 2 hr after injection with bovine serum albumin (BSA)-DTPA-In, galactosylated BSA-DTPA-In, goat antimouse IgG or avidin. Additionally, the effect of DTIn1 dose on 99mTc-DTPA abscess uptake was determined in a three-step protocol. The distribution of the radiolabels was studied by gamma counting of dissected tissue and gamma camera imaging. Priming with DTIn1 resulted in specific retention of 99mTc-DTPA in the abscess. Such 99mTc-DTPA abscess uptake was not dependent on the interval between the DTIn1 and the 99mTc-DTPA injection: Optimal 99mTc-DTPA abscess uptake was already achieved within a 2-hr time span between the DTIn1 and DTPA injections. However, relatively high 99mTc-DTPA backgro...Continue Reading

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