Rapid immunochromatographic test for detection of anti-factor XIII A subunit antibodies can diagnose 90 % of cases with autoimmune haemorrhaphilia XIII/13

Thrombosis and Haemostasis
Tsukaka OsakiA Ichinose

Abstract

Autoimmune haemorrhaphilia XIII/13 (AH13) is an acquired life-threatening bleeding disorder due to anti-factor XIII (FXIII) autoantibodies (auto-Abs). AH13 patients may die of haemorrhage without correct diagnosis and proper treatment because of lack of awareness and the absence of rapid easy-to-use tests specific for this disease. Currently, the definitive diagnosis is established by cumbersome and time-consuming laboratory tests such as dot-blot assays and enzyme-linked immunosorbent assays (ELISA), and therefore these tests are generally not carried out. To save AH13 patients' lives, there is an urgent necessity for developing a rapid test for FXIII auto-Abs. We first generated and characterised mouse monoclonal antibodies (mAb) against human FXIII A subunit (FXIII-A), and then developed a rapid immunochromatographic test (ICT) for detection of anti-FXIII-A auto-Abs using one mAb with a dissociation constant of 9.3 × 10⁻¹¹ M. The auto-Ab-FXIII-A complex was captured by the mAb on a nitrocellulose membrane and visualised by Au-conjugated anti-human IgG Ab. Mixing with healthy control plasma improved the detection of auto-Abs in patients having extremely low levels of FXIII-A. The specificity and sensitivity of the ICT were 87...Continue Reading

References

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Jan 1, 1996·Seminars in Thrombosis and Hemostasis·R EgbringR Seitz
Aug 28, 2007·Nature Methods·Jesper V OlsenMatthias Mann
Jul 26, 2008·Biochemistry·Masayoshi SouriAkitada Ichinose
Oct 1, 2010·Journal of Thrombosis and Haemostasis : JTH·I KomáromiL Muszbek
Jan 26, 2011·Thrombosis and Haemostasis·A IchinoseUNKNOWN Japanese collaborative research group on "Acquired haemorrha-philia due to factor XIII deficiency"
Aug 2, 2011·Seminars in Thrombosis and Hemostasis·Akitada Ichinose
Jul 1, 2011·Journal of Thrombosis and Haemostasis : JTH·H P KohlerUNKNOWN Factor XIII And Fibrinogen SSC Subcommittee Of The ISTH
Nov 28, 2012·Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis·Hiroyuki SugiyamaAkitada Ichinose
Feb 15, 2013·Thrombosis and Haemostasis·Hideho WadaAkitada Ichinose
Aug 10, 2013·Seminars in Thrombosis and Hemostasis·Verena Schroeder, Hans P Kohler

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Citations

Feb 21, 2018·Journal of Thrombosis and Haemostasis : JTH·L MuszbekÉ Katona
May 12, 2016·Haemophilia : the Official Journal of the World Federation of Hemophilia·M TsudaA Ichinose
Apr 14, 2021·Haemophilia : the Official Journal of the World Federation of Hemophilia·Akitada IchinoseMasayoshi Souri

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