Rapid inactivation of chloroplastic ascorbate peroxidase is responsible for oxidative modification to Rubisco in tomato (Lycopersicon esculentum) under cadmium stress

Journal of Integrative Plant Biology
Kai-Lang LiuJi-Ping Sheng

Abstract

To investigate the sensitive site of antioxidant systems in chloroplast under cadmium stress and its consequence on reactive oxygen species production and action, the sub-organellar localization of chloroplast superoxide dismutases (SOD, EC 1.15.1.1) and ascorbic peroxidase (APX, EC 1.11.1.11) isoenzymes and changes of enzymes activities under cadmium stress were investigated in tomato seedlings. Two APX isoforms, one thylakoid-bound and one stromal, were detected. Cd at 50 microM induced a moderate increase of SOD activities but a rapid inactivation of both APX isoenzymes. APX inactivation was mainly related to the decrease of ascorbate concentration, as supported by in vitro treatment of exogenous ascorbate and APX kinetic properties under Cd stress. H2O2 accumulation in chloroplast, as a consequence of APX inactivation, was associated with a 60% loss of Rubisco (EC 4.1.1.39) activity, which could be partially accounted for by a 10% loss of Rubisco content. Protein oxidation assay found that the Rubisco large subunit was the most prominent carbonylated protein; the level of carbonylated Rubisco large subunit increased fivefold after Cd exposure. Thiol groups in the Rubisco large subunit were oxidized, as indicated by non-redu...Continue Reading

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Citations

Jun 14, 2013·Omics : a Journal of Integrative Biology·Aïcha BelkadhiWided Chaïbi
Jan 10, 2012·Current Opinion in Plant Biology·Janine KönigKarl-Josef Dietz
Jul 20, 2011·Journal of Proteomics·Daniela BraconiAnnalisa Santucci
Sep 8, 2010·Ecotoxicology and Environmental Safety·Aline Finger-TeixeiraOsvaldo Ferrarese-Filho
Jul 8, 2010·Trends in Plant Science·Ian M Møller, Lee J Sweetlove
Sep 13, 2012·Journal of Integrative Plant Biology·Jinzhong CuiJoshua Fenn
Oct 16, 2014·Photosynthesis Research·Eugene A LysenkoVictor V Kusnetsov
Apr 28, 2021·Plant Science : an International Journal of Experimental Plant Biology·Wenshi HuJianwei Lu

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