Rapid increase in the partial pressure of NH3 on the cutaneous surface of air-exposed mangrove killifish, Rivulus marmoratus

The Journal of Experimental Biology
S L LitwillerP A Wright

Abstract

Mangrove killifish, Rivulus marmoratus, are tolerant of prolonged periods of air exposure (>30 days). Air-exposed R. marmoratus eliminate more than 40% of their total ammonia through NH(3) volatilization; however, the sites and mechanisms are unclear. We hypothesized that the cutaneous surface is an important site of NH(3) volatilization in air-exposed R. marmoratus. Ion-selective microelectrodes were used to measure the NH(4)(+) concentration and pH in the boundary layer on the cutaneous surface of fish in water or air (acute: 1 h, chronic: 11 days). Following acute and chronic air exposure, there was a approximately 18-fold increase in the NH(4)(+) concentration and a 0.3-0.6 pH unit increase on the cutaneous surface of R. marmoratus. In air-exposed fish, the calculated cutaneous partial pressure (P(NH(3))) was 608-1,251 microTorr, representing a 33- to 75-fold increase over control (immersed) fish. The P(NH(3)) on the cutaneous surface water film was more than sufficient to account for the rate of NH(3) volatilization under terrestrial conditions. Together, these data indicate that during air exposure, R. marmoratus utilize the cutaneous surface as a key site of NH(3) volatilization.

References

Feb 1, 1985·Biological Reviews of the Cambridge Philosophical Society·M E Feder, W W Burggren
Aug 1, 1965·Comparative Biochemistry and Physiology·T Berg, J B Steen
Jul 13, 2002·The Journal of Experimental Zoology·Michael Patrick Wilkie
Jan 30, 2004·The Journal of Experimental Biology·Shit F ChewYuen K Ip
Dec 8, 2004·The Journal of Experimental Biology·Dirk WeihrauchDavid W Towle

❮ Previous
Next ❯

Citations

May 11, 2013·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Chris N GloverChris M Wood
Feb 8, 2013·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christopher A CooperPatricia A Wright
Jan 17, 2008·The American Naturalist·D Scott TaylorBen B Chapman
Aug 23, 2012·Respiratory Physiology & Neurobiology·Patricia A Wright, Chris M Wood
Dec 20, 2011·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C A CooperP A Wright
Aug 25, 2015·Journal of Fish Biology·A J Turko, P A Wright
Aug 5, 2016·The Journal of Experimental Biology·Patricia A Wright, Andy J Turko
Aug 17, 2018·Proceedings. Biological Sciences·Michael D LivingstonPatricia A Wright
Apr 12, 2008·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Carrie C HungPatricia A Wright
Sep 25, 2017·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Quentin HeffellPatricia A Wright
Dec 11, 2020·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Patricia A Wright
Dec 18, 2020·The Journal of Experimental Biology·Yun-Wei DongAndrew Whitehead
Jul 21, 2009·The Journal of Experimental Biology·Patricia A Wright, Chris M Wood
Nov 26, 2011·The Journal of Experimental Biology·Michael P WilkieLeslie T Buck
May 19, 2009·The Journal of Experimental Biology·Dirk WeihrauchPatrick J Walsh
Dec 23, 2021·Proceedings. Biological Sciences·Megan R RidgwayPatricia A Wright

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.