Rapid induction of orthotopic hepatocellular carcinoma in immune-competent rats by non-invasive ultrasound-guided cells implantation.

BMC Gastroenterology
Hoi-Hung ChanMing-Hong Tai

Abstract

The fact that prognoses remain poor in patients with advanced hepatocellular carcinoma highlights the demand for suitable animal models to facilitate the development of anti-cancer medications. This study employed a relatively non-invasive approach to establish an orthotopic hepatocellular carcinoma model in immune-competent rats. This was done by ultrasound-guided implantation of cancer cells and the model was used to evaluate the therapeutic efficacy of short-term and low-dose epirubicin chemotherapy. Rat Novikoff hepatoma cells were injected percutaneously into the liver lobes of Sprague-Dawley rats under the guidance of high resolution ultrasound. The implantation rate and the correlation between dissected and ultrasound-measured tumor sizes were evaluated. A similar induction procedure was performed by means of laparotomy in a different group of rats. Pairs of tumor measurement were compared by ultrasound and computerized tomography scan. Rats with a successful establishment of the tumor were divided into the treatment (7-day low-dose epirubicin) group and the control group. The tumor sizes were non-invasively monitored by the same ultrasound machine. Blood and tumor tissues from tumor-bearing rats were examined by biochem...Continue Reading

References

Apr 19, 1989·Journal of the National Cancer Institute·R K Jain
Jan 1, 1994·Cold Spring Harbor Symposia on Quantitative Biology·R S KerbelC H Graham
May 23, 2002·Gastroenterology·Alex S Befeler, Adrian M Di Bisceglie
Oct 24, 2002·Journal of Clinical Gastroenterology·Hashem B El-Serag
Oct 24, 2002·Journal of Clinical Gastroenterology·Massimo Di MaioBruno Daniele
Feb 26, 2003·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jordi Bruix, Josep M Llovet
Jan 9, 2004·Japanese Journal of Clinical Oncology·Hiroshi IshiiTakayuki Hayashi
Mar 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Carolina ArmengolGabriel Capellá
Dec 3, 2005·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M ColleoniA Goldhirsch
Dec 24, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Jun YuGeoffrey C Farrell
Jan 8, 2008·The American Journal of Gastroenterology·Adriana SergioFabio Farinati
Nov 13, 2008·International Journal of Cancer. Journal International Du Cancer·Nathalie WongKa-Fai To
Feb 18, 2009·World Journal of Gastroenterology : WJG·Julien EdelineEveline Boucher
Oct 8, 2009·Dose-response : a Publication of International Hormesis Society·Jahangir Satti
Nov 3, 2009·The Lancet Oncology·Donald PoonUNKNOWN Asian Oncology Summit

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Citations

May 20, 2014·PloS One·Joris Tchouala Nofiele, Hai-Ling Margaret Cheng
Jan 24, 2012·Journal of Vascular and Interventional Radiology : JVIR·Scott M ThompsonDavid A Woodrum
Apr 12, 2014·Oncotarget·Tian-Huei ChuMing-Hong Tai

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Methods Mentioned

BETA
biopsy
X-ray
transfection

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