Rapid Intraoperative Diagnosis of Pediatric Brain Tumors Using Stimulated Raman Histology

Cancer Research
Todd HollonDaniel A Orringer

Abstract

Accurate histopathologic diagnosis is essential for providing optimal surgical management of pediatric brain tumors. Current methods for intraoperative histology are time- and labor-intensive and often introduce artifact that limit interpretation. Stimulated Raman histology (SRH) is a novel label-free imaging technique that provides intraoperative histologic images of fresh, unprocessed surgical specimens. Here we evaluate the capacity of SRH for use in the intraoperative diagnosis of pediatric type brain tumors. SRH revealed key diagnostic features in fresh tissue specimens collected from 33 prospectively enrolled pediatric type brain tumor patients, preserving tumor cytology and histoarchitecture in all specimens. We simulated an intraoperative consultation for 25 patients with specimens imaged using both SRH and standard hematoxylin and eosin histology. SRH-based diagnoses achieved near-perfect diagnostic concordance (Cohen's kappa, κ > 0.90) and an accuracy of 92% to 96%. We then developed a quantitative histologic method using SRH images based on rapid image feature extraction. Nuclear density, tumor-associated macrophage infiltration, and nuclear morphology parameters from 3337 SRH fields of view were used to develop and ...Continue Reading

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Citations

Jul 1, 2020·CNS Oncology·Siri Sahib S KhalsaDaniel A Orringer
May 12, 2020·Molecular & Cellular Oncology·Todd C Hollon, Daniel A Orringer
Jul 7, 2018·Journal of Healthcare Engineering·Sishan CuiShuhua Yue
Dec 21, 2018·Cancer Metastasis Reviews·Gregory W AunerMichelle A Brusatori
Jun 14, 2019·Frontiers in Surgery·Nikita Lakomkin, Constantinos G Hadjipanayis
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Jan 15, 2021·Chemistry, an Asian Journal·Adukkadan N RamyaKaustabh K Maiti
Feb 22, 2021·Journal of Neuro-oncology·Todd Hollon, Daniel A Orringer
Mar 9, 2021·World Neurosurgery·Long DiSakir H Gultekin
May 10, 2021·Trends in Molecular Medicine·Nina OgrincIsabelle Fournier
Apr 19, 2020·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Suman B MondalSamuel Achilefu
Jun 4, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Swati TanwarIshan Barman
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