Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein.

Nature Medicine
Seth J ZostJames E Crowe

Abstract

Antibodies are a principal determinant of immunity for most RNA viruses and have promise to reduce infection or disease during major epidemics. The novel coronavirus SARS-CoV-2 has caused a global pandemic with millions of infections and hundreds of thousands of deaths to date1,2. In response, we used a rapid antibody discovery platform to isolate hundreds of human monoclonal antibodies (mAbs) against the SARS-CoV-2 spike (S) protein. We stratify these mAbs into five major classes on the basis of their reactivity to subdomains of S protein as well as their cross-reactivity to SARS-CoV. Many of these mAbs inhibit infection of authentic SARS-CoV-2 virus, with most neutralizing mAbs recognizing the receptor-binding domain (RBD) of S. This work defines sites of vulnerability on SARS-CoV-2 S and demonstrates the speed and robustness of advanced antibody discovery platforms.

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Methods Mentioned

BETA
transfection
ELISA
electron microscopy
flow cytometry
single-cell sequencing
X-ray
PCR
leukapheresis
size-exclusion chromatography
chips

Software Mentioned

GraphPad
SH800
GENEWIZ
InCell Analyzer 1000 Workstation
cryoSPARC
RTCA HT
PyIR
NEBuilder
Genomics Cell Ranger
10x

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