Rapid isolation and profiling of a diverse panel of human monoclonal antibodies targeting the SARS-CoV-2 spike protein.

Nature Medicine
Seth J ZostJames E Crowe


Antibodies are a principal determinant of immunity for most RNA viruses and have promise to reduce infection or disease during major epidemics. The novel coronavirus SARS-CoV-2 has caused a global pandemic with millions of infections and hundreds of thousands of deaths to date1,2. In response, we used a rapid antibody discovery platform to isolate hundreds of human monoclonal antibodies (mAbs) against the SARS-CoV-2 spike (S) protein. We stratify these mAbs into five major classes on the basis of their reactivity to subdomains of S protein as well as their cross-reactivity to SARS-CoV. Many of these mAbs inhibit infection of authentic SARS-CoV-2 virus, with most neutralizing mAbs recognizing the receptor-binding domain (RBD) of S. This work defines sites of vulnerability on SARS-CoV-2 S and demonstrates the speed and robustness of advanced antibody discovery platforms.


Oct 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Boyd YountRalph S Baric
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Jianhua SuiWayne A Marasco
Apr 23, 2004·Biological Procedures Online·Melanie OhiThomas Walz
Jun 29, 2004·Lancet·Jan ter MeulenAlbert D M E Osterhaus
Sep 27, 2005·Journal of Structural Biology·David N Mastronarde
Jul 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Zhongyu ZhuDimiter S Dimitrov
Jan 12, 2011·The Journal of Experimental Medicine·Jens WrammertPatrick C Wilson
Sep 18, 2013·Proceedings of the National Academy of Sciences of the United States of America·Trevor ScobeyRalph S Baric
Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Xian-Chun TangWayne A Marasco
Jul 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Davide CortiAntonio Lanzavecchia
Jul 16, 2016·Virology·Swati MukherjeeTheodore C Pierson
Feb 7, 2017·Nature Methods·Ali PunjaniMarcus A Brubaker
Dec 13, 2018·Methods in Molecular Biology·Jenna J GuthmillerPatrick C Wilson
Feb 8, 2019·Journal of Virology·Lauren E WilliamsonJames E Crowe
Jan 25, 2020·The New England Journal of Medicine·Na ZhuUNKNOWN China Novel Coronavirus Investigating and Research Team
Feb 1, 2020·The New England Journal of Medicine·Michelle L HolshueUNKNOWN Washington State 2019-nCoV Case Investigation Team
Feb 23, 2020·Science·Daniel WrappJason S McLellan
Mar 11, 2020·Cell·Alexandra C WallsDavid Veesler
Apr 7, 2020·Trends in Immunology·Shibo JiangLanying Du
Jun 20, 2020·Nature·Davide F RobbianiMichel C Nussenzweig
Aug 5, 2020·Nature Biomedical Engineering·Pavlo GilchukRobert H Carnahan
May 12, 2020·BioRxiv : the Preprint Server for Biology·P. BrouwerMarit van Gils

❮ Previous
Next ❯


Sep 18, 2020·Expert Opinion on Therapeutic Targets·Shibo JiangLanying Du
Aug 14, 2020·Nature·Mark J MulliganKathrin U Jansen
Sep 4, 2020·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Pratip K ChattopadhyayUNKNOWN other members of the ISAC COVID-19 Work Group
Sep 24, 2020·Signal Transduction and Targeted Therapy·Fei YuShibo Jiang
Jul 24, 2020·Nature Structural & Molecular Biology·Rory HendersonPriyamvada Acharya
Aug 5, 2020·Nature Biomedical Engineering·Pavlo GilchukRobert H Carnahan
Oct 13, 2020·Nature·Christopher O BarnesPamela J Bjorkman
Nov 20, 2020·The Journal of Experimental Medicine·Alexandra SchäferTimothy P Sheahan
Nov 25, 2020·Current Opinion in HIV and AIDS·Pilar MendozaChristian Gaebler
Nov 21, 2020·The Journal of Clinical Investigation·Andrew I Flyak
Jan 14, 2021·Journal of Biomedical Science·Ioannis P TrougakosMeletios A Dimopoulos
Jan 28, 2021·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·George LagoumintzisKonstantinos Poulas
Dec 21, 2020·Stem Cell Research·Shi XiaojieQiang Min
Dec 31, 2020·Journal of Molecular Cell Biology·Liu Daisy LiuFei-Long Meng
Feb 12, 2021·Cell·Aurélien SokalMatthieu Mahévas
Feb 17, 2021·EClinicalMedicine·Seiya YamayoshiYoshihiro Kawaoka
Dec 3, 2020·Frontiers in Cellular and Infection Microbiology·Rinki MinakshiJihoe Kim
Nov 4, 2020·Proceedings of the National Academy of Sciences of the United States of America·Wei LiDimiter S Dimitrov
Nov 13, 2020·Frontiers in Pharmacology·Junqing HuangJia-Xu Chen
Dec 12, 2020·Scientific Reports·John V DzimianskiRebecca M DuBois
Feb 17, 2021·Biomedical Journal·Juan Pablo Jaworski
Jan 22, 2021·Nature Communications·Zhiqiang KuZhiqiang An
Oct 19, 2020·Biochemical and Biophysical Research Communications·Meng YuanIan A Wilson
Feb 2, 2021·Nature·Annette B VogelUgur Sahin
Feb 16, 2021·Protein Science : a Publication of the Protein Society·Benjamin N BellPeter S Kim
Feb 25, 2021·Science Immunology·Mrunal SakharkarLaura M Walker
Mar 3, 2021·Journal of Perinatology : Official Journal of the California Perinatal Association·Veronique Demers-MathieuElena Medo
Mar 7, 2021·Vaccines·Emily Bettini, Michela Locci

❮ Previous
Next ❯

Methods Mentioned

electron microscopy
flow cytometry
single-cell sequencing
size-exclusion chromatography

Software Mentioned

InCell Analyzer 1000 Workstation
Genomics Cell Ranger

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.